AM33331PU-S Myeloid specific antigen (Myeloid Cell Marker) antibody

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0.1 mg / €320.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human Myeloid specific antigen BM-1

Product Description for Myeloid specific antigen

Mouse anti Human Myeloid specific antigen BM-1.
Properties: (Myeloid Cell Marker)
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Properties for Myeloid specific antigen

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, P
Clonality Monoclonal
Clone BM-1
Host Mouse
Isotype IgG1
Molecular weight 183kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human peripheral blood mononuclear cells.
Property Myeloid Cell Marker
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow Cytometry: 0.5-1 µg/million cells. 
Immunofluorescence: 1-2 µg/ml. 
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml. 
and Formalin-Fixed Paraffin Sections:
 0.5-1 µg/ml. 
Incubation Time: 30 minutes at RT. 
No special pretreatment is required for staining of formalin/paraffin tissues.
Positive Control: Bone marrow, lymph node or tonsil.
Background BM-1 antigen is a 183 kD myeloid-specific-DNA-binding protein which is expressed in myeloid cells, including myeloid precursors and mature granulocytes. It is also expressed in chronic myeloid leukemia (CML) and M2 and M3 acute myeloid leukemias (AML) as well as myelomonocytic leukemias, but not in T, B, NK and other non-hematopoietic cells. The biological function of this antigen is not clear, although it has been proposed that BM-1 may play a role in the differentiation of myeloid cells.
Concentration 0.2 mg/ml
General Readings
  1. Epstein AL, Samoszuk M, Stathopoulos E, Naeve GS, Clevenger CV, Weil S, et al. Immunohistochemical characterization of a 183 KD myeloid-specific-DNA-binding protein in B5 fixed, paraffin-embedded tissues, and bone marrow aspirates by monoclonal antibody BM-1. Blood. 1987 Oct;70(4):1124-30. PubMed PMID: 3307947.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
Recognizes 183kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen.  BM-1 reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. BM-1 and BM-2 antibodies react with early precursor and mature forms of human myeloid cells.
BM-1 Monoclonal Antibody is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that BM-1 may play a role in the differentiation of myeloid cells.
Cellular Localization: Cytoplasmic and Nuclear.
Species:
Human.

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