NB200-593 Myosin antibody

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Mouse anti Bovine, Human Myosin SM-M5

Product Description for Myosin

Mouse anti Bovine, Human Myosin SM-M5.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections, Western blot / Immunoblot.

Properties for Myosin

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Purified
Reactivity Bov, Hu
Applications C, P, WB
Clonality Monoclonal
Clone SM-M5
Host Mouse
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Crude human uterus extract.
Application immunofluorescence, Immunohistochemistry-Paraffin, IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 2-4ug/ml for 30 minutes at RT. Staining of formalin fixed tissues REQUIRES digesting sections with typsin at 1mg/ml PBS, 10 min at 37 degrees C, followed by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Not tested in any other applications. Optimal dilutions should be determined by the end user., immunofluorescence , Immunohistochemistry-Paraffin 2-4ug/ml
Background SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.
Concentration 0.2 mg/ml
General Readings
  1. Frid MG, et al. Dev Biol 1992; 153:185.
  2. Glukhova MA, et al. J Biol Chem 1990; 265(22):13042.
Storage Store at 4C. Do not freeze.
Tissue culture supernatant
Buffer System:
0.09% Sodium Azide
Tissue culture supernatant
This reacts with Myosin heavy chain 1 and 2. Detects a band at 205 kDa (MHC-1) and 200 kDa (MHC-2)
Bovine, Human

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