DM207S Myosin (skeletal muscle) antibody

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Mouse anti Bovine, Chicken, Guinea Pig, Human, Mouse, Rabbit, Rat Myosin (skeletal muscle) MY-32


Product Description for Myosin (skeletal muscle)

Mouse anti Bovine, Chicken, Guinea Pig, Human, Mouse, Rabbit, Rat Myosin (skeletal muscle) MY-32.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Myosin (skeletal muscle)

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Supernatant
Reactivity Bov, Chk, GP, Hu, Ms, Rb, Rt
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone MY-32
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Rabbit muscle myosin
Mol. Weight of Antigen: 480 kD

Format: This antibody is supplied as liquid, supernatant in 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.
Application Immunohistochemistry (Paraffin/Frozen; 1:50-1:100), Western Blot (1:100-1:500; Recommended positive control: Skeletal muscle tissue), Indirect Immunofluorescence 1:50-1:100. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.4 mg/ml IgG
General Readings
  1. Havenith, M,G; et al; Muscle fiber typingin routinely procesed skeletal musclewith monoclonal an
Storage Store the antibody at 4°C. Do not freeze!
Do not dilute the entire solution at once since the diluted solution may have reduced stability. In general, 0.05 M Borate, pH 8.0, containing 0.15 M Sodium Chloride, 0.05 % Sodium Azide, is a good diluent to use with most antibodies. Shelf life: one year from despatch.
Specific for the myosin heavy chain using an immunoblotting technique with either human or animal skeletal muscle. This antibody localizes an epitope on the myosin chain that is stable to the routine formalin fixation. Anti-skeletal myosin to fast twitch skeletal myosin may be used for detecting cross-striated muscle differentiation in tumors.
This antibody stains fast type II and neonatal isomyosin molecules found in skeletal muscle and does not stain cardiac muscle, smooth muscle or
non-muscle, and human rhabdomyosarcomas.
Species reactivity: Human, rabbit, bovine, feline, chicken, guinea pig, mouse, rat. Others not tested. Cellular localization: cytoplasmic.

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