AM10205PU-N N epsilon-(gamma-L-glutamyl)-L-lysine isopeptide antibody

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Mouse anti All Species N epsilon-(gamma-L-glutamyl)-L-lysine isopeptide 81D4

Product Description for N epsilon-(gamma-L-glutamyl)-L-lysine isopeptide

Mouse anti All Species N epsilon-(gamma-L-glutamyl)-L-lysine isopeptide 81D4.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Enzyme Immunoassay.

Properties for N epsilon-(gamma-L-glutamyl)-L-lysine isopeptide

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity All Species
Applications C, E, ICC/IF
Clonality Monoclonal
Clone 81D4
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Ne-(g-L-glutamyl)-L-lysine isopeptide
Isotype control SM13P
Application ELISA. 

Immunohistochemistry: 1/50-1/800.
Concentration 1.0 mg/ml (after reconstitution)
General Readings
  1. Shaik KS, Meyer F, Vázquez AV, Flötenmeyer M, Cerdán ME, Moussian B. δ-Aminolevulinate synthase is required for apical transcellular barrier formation in the skin of the Drosophila larva. Eur J Cell Biol. 2012 Mar;91(3):204-15. doi: 10.1016/j.ejcb.2011.11.005. Epub 2012 Jan 30. PubMed PMID: 22293958.
  2. Nadalutti CA, Korponay-Szabo IR, Kaukinen K, Griffin M, Mäki M, Lindfors K. Celiac disease patient IgA antibodies induce endothelial adhesion and cell polarization defects via extracellular transglutaminase 2. Cell Mol Life Sci. 2014 Apr;71(7):1315-26. doi: 10.1007/s00018-013-1455-5. Epub 2013 Aug 28. PubMed PMID: 23982754.
  3. de Jager M, van der Wildt B, Schul E, Bol JG, van Duinen SG, Drukarch B, Wilhelmus MM. Tissue transglutaminase colocalizes with extracellular matrix proteins in cerebral amyloid angiopathy. Neurobiol Aging. 2012 Oct 30.
  4. Elli L, Ciulla MM, Busca G, Roncoroni L, Maioli C, Ferrero S, et al. Beneficial effects of treatment with transglutaminase inhibitor cystamine on the severity of inflammation in a rat model of inflammatory bowel disease. Lab Invest. 2011 Mar;91(3):452-61. doi: 10.1038/labinvest.2010.186. Epub 2010 Nov 1. PubMed PMID: 21042292.
  5. T homas V et al. Definition of the fine specificity of the monoclonal antibody 81D4: its reactivity with lysine and polyamine isopeptide cross-links. J Immunol Methods. 2004 Sep:292(1-2):83-95.
  6. Johnson GV, LeShoure R Jr. Immunoblot analysis reveals that isopeptide antibodies do not specifically recognize the epsilon-(gamma-glutamyl)lysine bonds formed by transglutaminase activity. J Neurosci Methods. 2004 Apr 30:134(2):151-8.
  7. Kaartinen MT, El-Maadawy S, Räsänen NH, McKee MD. Tissue transglutaminase and its substrates in bone. J Bone Miner Res. 2002 Dec;17(12):2161-73. PubMed PMID: 12469910.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
20mM PO4, 1M NaCl, 2% Sucrose
Lyophilized purified powder
Restore in distilled water.
Reacts with Ne (g-L-glutamyl)-L-lysine.
Cross reacts in ELISA with N1,N4 bis (gPoly L-glutamyl) Putrescine and N1,N8 bis (gPoly L-glutamyl) Spermidine.
There is no cross reaction with: Protein bound Ne acetyl lysine, protein bound N1 or N4 mono (gPoly L-glutamyl) Putrescine, protein bound N1 mono (gPoly L-glutamyl) Spermidine, protein bound Ne (g -L-glutamyl) lysine having a free alpha amine either on its glutamyl or lysyl moieties.
The ability of this antibody to fix complement is unknown.

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