discontinued

AP16396PU-N NANOG (144-157) antibody

See related secondary antibodies

Search for all "NANOG"

Quick Overview

Goat anti Canine, Human NANOG

Product Description for NANOG

Goat anti Canine, Human NANOG.
Properties: (144-157)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for NANOG

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Homeobox protein NANOG
Presentation Aff - Purified
Reactivity Can, Hu
Applications E, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9H9S0
Immunogen:
Peptide from the internal region of the protein sequence according to NP_079141.2.
AA Sequence:
C-QNQRMKSKRWQKNN
GeneID:
79923
Property 144-157
Application Peptide ELISA: 1/64000 (Detection Limit). 
Western blot: 0.03-0.1 µg/ml. Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1).
Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). (See also Ref.2)
This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Background Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.
NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Montserrat N, Ramírez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, et al. Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2. J Biol Chem. 2012 Jul 13;287(29):24131-8. doi: 10.1074/jbc.M112.350413. Epub 2012 May 21. PubMed PMID: 22613719. (Free PMC Article available, 3 images available)
  2. Vassena R, Montserrat N, Carrasco Canal B, Aran B, de Oñate L, Veiga A, et al. Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells. Hum Mol Genet. 2012 Aug 1;21(15):3366-73. doi: 10.1093/hmg/dds168. Epub 2012 Apr 30. PubMed PMID: 22547223. (Free PMC Article available, 3 images available)
  3. Raya A, Rodríguez-Pizà I, Guenechea G, Vassena R, Navarro S, Barrero MJ, et al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature. 2009 Jul 2;460(7251):53-9. doi: 10.1038/nature08129. Epub 2009 May 31. PubMed PMID: 19483674. (Free PMC Article available, 5 images available)
  4. Montserrat N, Garreta E, González F, Gutiérrez J, Eguizábal C, Ramos V, et al. Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system. J Biol Chem. 2011 Apr 8;286(14):12417-28. doi: 10.1074/jbc.M110.168013. Epub 2011 Feb 1. PubMed PMID: 21285354. (Free PMC Article available, 9 images available)
  5. Montserrat N, Bahima EG, Batlle L, Häfner S, Rodrigues AM, González F, et al. Generation of pig iPS cells: a model for cell therapy. J Cardiovasc Transl Res. 2011 Apr;4(2):121-30. doi: 10.1007/s12265-010-9233-3. Epub 2010 Nov 19. PubMed PMID: 21088946.
  6. Rodríguez-Pizà I, Richaud-Patin Y, Vassena R, González F, Barrero MJ, Veiga A, et al. Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions. Stem Cells. 2010 Jan;28(1):36-44. doi: 10.1002/stem.248. PubMed PMID: 19890879.
  7. Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, et al. Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol. 2008 Nov;26(11):1276-84. doi: 10.1038/nbt.1503. Epub 2008 Oct 17. PubMed PMID: 18931654.
General Readings 1. Raya A et.al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature. 2009 Jul 2;460(7251):53-9. PMID: 19483674
2. Montserrat N, Garreta E, González F, Gutiérrez J, Eguizábal C, Ramos V, Borrós S, Izpisua Belmonte JC. Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system.
J Biol Chem. 2011 Apr 8;286(14):12417-28. PMID: 21285354
3. Montserrat N, Bahima EG, Batlle L, Häfner S, Rodrigues AM, González F, Belmonte JC.
Generation of pig iPS cells: a model for cell therapy. J Cardiovasc Transl Res. 2011 Apr;4(2):121-30. PMID: 21088946
4. Rodríguez-Pizà I, Richaud-Patin Y, Vassena R, González F, Barrero MJ, Veiga A, Raya A, Belmonte JC. Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions.
Stem Cells. 2010 Jan;28(1):36-44. PMID: 19890879
5. Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Izpisúa Belmonte JC. Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol. 2008 Nov;26(11):1276-84. PMID: 18931654
6. Montserrat N, Ramírez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, González F, Izpisua Belmonte JC, Campistol JM. Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2. J Biol Chem. 2012 Jul 13;287(29):24131-8. PMID: 22613719
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH~7.3
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Canine (Dog).
Species reactivity (tested):
Human, Pig.
Specificity
Specificity:
This antibody is specific to NANOG.
Gene ID 79923
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for NANOG (17 products)

Catalog No. Species Pres. Purity   Source  

NANOG

NANOG Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 20 µg / €199.00

Special Price: 20 µg / €139.00

  OriGene Technologies, Inc.

NANOG

NANOG Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 20 µg / €199.00

Special Price: 20 µg / €139.00

  OriGene Technologies, Inc.

NANOG (+C-term TAT peptide)

NANOG Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses E. coli
  Acris Antibodies GmbH

NANOG (+C-term TAT peptide)

NANOG Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses E. coli
  Acris Antibodies GmbH

NANOG

NANOG Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  Acris Antibodies GmbH

NANOG

NANOG Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  Acris Antibodies GmbH

NANOG (1-154, His-tag)

NANOG Human Purified > 90 % E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

NANOG (1-154, His-tag)

NANOG Human Purified > 90 % E. coli
50 µg / €370.00
  Acris Antibodies GmbH

NANOG (1-305)

NANOG Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

NANOG (1-305)

NANOG Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

NANOG

NANOG Human
  Abnova Taiwan Corp.

NANOG

NANOG Human
  Abnova Taiwan Corp.

NANOG

NANOG Human
  Abnova Taiwan Corp.

NANOG

NANOG Human
  Abnova Taiwan Corp.

NANOG

NANOG Human
  Abnova Taiwan Corp.

NANOG

NANOG Human
  Abnova Taiwan Corp.

NANOG

Western Blot: Nanog Protein [NBP1-42463] - This protein was tested using the anti-Nanog antibody NB100-93546.  500 ng of protein was used on the western blot and detected with NB100-93546. Human Purified
  Novus Biologicals Inc.

Positive controls for NANOG (1 products)

Catalog No. Species Pres. Purity   Source  

Nanog Lysate

Western Blot: Nanog Lysate [NBL1-13465] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for NANOG
  Novus Biologicals Inc.
  • LinkedIn