AP00492PU-N Nck antibody

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Rabbit anti Human, Mouse, Rat Nck


Product Description for Nck

Rabbit anti Human, Mouse, Rat Nck.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Nck

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms DP3088
Presentation Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Full length recombinant human NCK protein.
Application Immunofluorescence and Immunoprecipitation (Denatured): Use Protein A. Ab at 10 µg/mg protein lysate. Western Blotting: 5-10 µg/ml for 2hrs at RT. Recommended positive control: A431 Cells.
Background Nck is a highly conserved protein that has one SH2 and three SH3 domains. It is ubiquitously expressed in mammalian tissues. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation by EGF and PDGF. Nck has been proposed to function as an adaptor protein by coupling protein tyrosine kinases to signaling pathways that lead to cell proliferation. Overexpression of Nck results in transformation of NIH 3T3 cells.
Concentration 1 mg/ml
General Readings
  1. Birge RB; et al. Genes Cells, 1996, 1:595-613.
  2. Kitamura Y; et al. Biochemical J, 1997, 322:873-8.
  3. Lawe DC; et al. Oncogen, 1997, 14(2):223-31.
Storage Store at 4°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles!
Shelf life: one year from despatch.
Protein A affinity purified immunoglobulin fraction
Buffer System:
10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer.
Highly specific for Nck. Cellular Localization: Cytoplasmic.
Mol. Wt of Antigen: 47kD.
Human, Mouse, and Rat.

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