DM3152 Nck antibody

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Mouse anti Human Nck 20B-1H9


Product Description for Nck

Mouse anti Human Nck 20B-1H9.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for Nck

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications IP, WB
Clonality Monoclonal
Clone 20B-1H9
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Recombinant human Nck protein.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunoprecipitation. Western Blot. Recommended positive control: A431 Cells. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings 1. Birge RB; et al. Genes Cells, 1996 , 1:595-613.
2. Kitamura Y; et al. Biochemical J, 1997, 322:873-8.
3. Lawe DC; Hahn C; Wong AJ. The Nck SH2/SH3 adaptor protein is present in the nucleus and associates with the nuclear protein SAM68. On cogene, 1997 Jan 16, 14(2):223- 4
4. Lu W; Katz S; Gupta R; Mayer BJ. Activation of Pak by membrane localization mediated by an SH3 domain from the adaptor protein Nck. Current Biology, 1997 Feb 1, 7(2):85- 5
5. Lussier G; Larose L. A casein kinase I activity is constitutively associated with Nck. Journal of Biological Chemistry, 1997 Jan 31, 272(5):2688-94.
6. Morimoto C; Tachibana K. Beta 1 integrin-mediated signalling in human T cells. Human Cell, 1996, 9(3):163-8.
7. Park D. Cloning, sequenc ing, and overexpression of SH2/SH3 adaptor protein Nck from mouse thymus. Molecules and Cells, 1997, 7(2):231-6.
8. Schlaepfer DD; Broome MA; Hunter T. Fibronectin stimulated signaling from a focal adhesion kinase-c-Src complex: involvement of the Grb2, p130cas, and Nck adaptor proteins. Molecular and Cellular Biology, 1997
Mar, 17(3):1702-13.
9. Su YC; Han J; Xu S; Cobb M; Skolnik EY. NIK is a new Ste20-related kinase that binds NCK and MEKK1 and activates the SAPK/JNK cascade via a conserved regulatory domain. Embo Journal, 1997, 16(6):1279-90.
10. Tanaka M; Lu W; Gupta R; Mayer BJ. Expression of mutated Nck SH2/SH3 adaptor respecifies mesodermal cell fate in Xenopus laevis devel opment. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(9):4493-8.
11. Bokoch GM; Wang Y; Bohl BP; Sells MA; Quilliam LA; Knaus UG. Interaction of the Nck adapter protein with p21-activated kinase (PAK1). Journal of Biological Chemistry, 1996, 271:25746-9.
12. Choudhury GG; Marra F; Abboud HE. Thrombin stimulates association of src homology domain containing adaptor protein Nck with pp125FAK. Am J Physiol, 1996, 270:F295-300.
13. Galisteo ML; Chernoff J; Su YC; Skolnik EY; Schlessinger J. The adaptor protein Nck li nks receptor tyrosine kinases with the serine-threonine kinase Pak1. Journal of Biological Chemistry, 1996 Aug 30, 271(35):20997-1000.
14. Kochhar KS; Iyer AP. Hepatocyte growth factor induces activation of Nck and phospholipase C-gamma in lung carcinoma cells. Cancer Letters, 1996 Jul 12, 104(2):163-9.
15. Quilliam LA; Lambert QT; Mickelson-Young LA; Westwick JK; Sparks AB; Kay BK; Jenkins NA; Gilbert DJ; Copeland NG; Der CJ. Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and poten tial effector of Rho protein signaling. Journal of Biol ogical Chemistry,
1996 Nov 15, 271(46):28772-6.
16. Roche S; McGlade J; Jones M; Gish GD; Pawson T; Courtneidge SA. Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent a nd Ras-independent
pathways. Embo Journal, 1996 Sep 16, 15(18):4940-8.
17. Rockow S; Tang J; Xiong W; Li W. Nck inhibits NGF and basic FGF induced PC12 cell differentiation via mitogenactivated protein kinase-indepe ndent pathway. Oncogene, 1996, 12:2351-9.
18. Chou MM; Hanafusa H. A novel ligand for SH3 domains. The Nck adaptor protein binds to a serine/threonine kinase via an SH3 domain. Journal of Biological Chemistry, 1995, 270(13):7359-64.
19. Hu Q; Milfay D; Williams LT. Binding of NCK to SOS and activation of ras-dependent gene expression. Molecular and Cellular Biology, 1995 Mar, 15(3):1169-74.
20. Rivero-Lezcano OM; Marcilla A; Sameshima JH; Robbins KC. Wiskott-Aldrich syndrome protein physically associates with Nck through Src homology 3 domains. Molecular and Cellular Biology, 1995 Oct, 15(10):5725-31.
21. Tanaka M; Gupta R; Mayer BJ. Differential inhibition of signaling pathways by dominant-negative SH2/SH3 adapter proteins. Molecular and Cellular Biology, 1995 Dec, 15(12):6829-37.
22. Lee CH; Li W; Nishimura R; Zhou M; Batzer AG; Myers MG Jr; White MF; Schlessinger J; Skolnik EY. Nck associates with the SH2 domain-docking protein IRS-1 in insulin-stimulated cells. Pr oceedings of the National Academy of Sciences of the Un ited States of America,
1993, 90(24):11713-7.
23. Chou MM; Fajardo JE; Hanafusa H. The SH2- and SH3-containing Nck protein transfor ms mammalian fibroblasts in the absence of elevated phosphot yrosine levels. Molecular and Cellular Biology, 1992 Dec, 12(12):5834-42.
24. Li W; Hu P; Skolnik EY; Ullrich A; Schlessinger J. The SH2 and SH3 domain-containing Nck protein is oncogenic and a common target for phosphorylation by different surface receptors. Molecular an
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Nck is a highly conserved protein that has one SH2 and three SH3 domains. It is ubiquitously expressed in mammalian tissues. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation by EGF and PDGF. Cellular Localization: cytoplasmic.

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