DP3088 Nck antibody

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Rabbit anti Human, Mouse, Rat Nck


Product Description for Nck

Rabbit anti Human, Mouse, Rat Nck.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Nck

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Full length recombinant human NCK protein.
Application Immunofluorescence. Immunoprecipitation (Denatured verified): Use Protein A. Ab at 10µg/mg protein lysate. Western Blotting: 5-10µg/ml for 2hrs at RT. Recommended positive control: A431 Cells. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 1 mg/ml
General Readings
  1. Birge RB; et al. Genes Cells, 1996, 1:595-613.
  2. Kitamura Y; et al. Biochemical J, 1997, 322:873-8.
  3. Lawe DC; et al. Oncogen
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Nck is a highly conserved protein that has one SH2 and three SH3 domains. It is ubiquitously expressed in mammalian tissues. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation by EGF and PDGF. Reacts with Human, Mouse, and Rat. Others not tested. Cellular Localization: Cytoplasmic.

Mol. Wt of Antigen: 47kD.

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