AP32375SU-N NDEL antibody

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Mouse anti Rat NDEL

Product Description for NDEL

Mouse anti Rat NDEL.
Presentation: Serum
Product is tested for Western blot / Immunoblot.

Properties for NDEL

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms EOPA, MITAP1, NUDEL, Nuclear distribution protein nudE-like 1
Presentation Serum
Reactivity Rt
Applications WB
Clonality Polyclonal
Host Mouse
Molecular weight ~38 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Synthetic Linear Peptide.
Application Western Blot: 1/5000.
Control: Brain Lysate.
Background NudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NUDEL), also known as NDEL1, is a human gene. This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. This protein plays a role in nervous system development and is linked to LIS-1, the disease gene for a form of lissencephaly, a disorder of cortical development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Protocols Western Blot
1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.
2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.
3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)
4. Stain the transferred bands with Millipore BLOT-FastStain.
5. Destain with deionized water.
6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. Th e antibody preparation should be centrifuged before use (10,000 g for 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.
8. Wash 4 x 10 min. with PBS-0.1% Tween 20. From this stage, azide should be omitted.
9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, diluted appropriately) 1 h at room temperature.
10. Wash 4 x 10 min. with PBS-0.1% tween 20.
11. Perform ECL with commercial kits.
General Readings
  1. Hayashi, M. et al. (2005) PNAS. 10: 3828-3833
Storage Store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
0.05% Sodium Azide
Liquid Serum
Recognizes Rat NUDEL. 
Other species not tested.

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