NB100-78493 NEK2 antibody

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Mouse anti Human NEK2 8A6

Product Description for NEK2

Mouse anti Human NEK2 8A6.
Presentation: Biotin
Product is tested for Western blot / Immunoblot.

Properties for NEK2

Product Category Primary Antibodies
Quantity 0.25 mg
Presentation Biotin
Reactivity Hu
Applications WB
Clonality Monoclonal
Clone 8A6
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Recombinant protein
Isotype control SM10B (for use in human samples)
Application Western Blot
Background NEK2 is a serine/threonine kinase involved in mitotic regulation. NEK2 also known as serine/threonine protein kinase Nek2, NimA-related protein kinase 2, and HSPK21, can be expressed as 3 different isoforms as a result of alternative splicing.. The predicted molecular weight of full-length protein is approximately 52 kD. Isoform 1 and isoform 2 forms homo- and heterodimers; isoform 1 has a nucleolar targeting/retention signal in C-terminal domain. Isoform 2 is missing the C-terminal sequence and remains mainly in the cytoplasm. Isoform 1 plays a role in centrosome splitting and phosphorylates and activates NEK11 is G1/S arrested cells. NEK2 accumulates throughout S phase, becoming maximal in late G2 with expression similar to that of A and B cyclins. Isoform 1 is not present in cells arrested in G2/M prometaphase. Both isoform 1 and 2 are expressed in peripheral T cells and many other transformed cell types. The NEK2 protein has been shown to interact with the TERF1 protein. The 8A6 monoclonal antibody recognizes human NEK2 and is useful for Western blotting.
General Readings
  1. Schultz, S., et al., 1994. Cell Growth Differ. 5:625.2. Hames, R.S., and Fry, A.M. 2002. Biochem J. 361:77.3. Noguchi, K., et al., 2004. J. Biol. Chem. 279:32716.
Storage Store at 4C. Do not freeze. Do not freeze.
Immunogen affinity purified
Buffer System:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0
Immunogen affinity purified
Human (does not cross-react with mouse).

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