AM50500C3-N NeuN antibody

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Mouse anti Mouse, Rat NeuN A60

Product Description for NeuN

Mouse anti Mouse, Rat NeuN A60.
Presentation: Cy3
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for NeuN

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms NEUronal Nuclei, Neuronal Nuclei
Presentation Cy3
Reactivity Ms, Rt
Applications ICC/IF
Clonality Monoclonal
Clone A60
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Purified cell nuclei from mouse brain.
Application Immunocytochemistry: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells..
Background NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
Concentration lot-specific
Storage Store undiluted at 2-8°C.
Shelf life: One year from despatch.
Protein A Chromatography
Buffer System:
PBS with 0.09% sodium azide and 15 mg/mL BSA.
Liquid purified Ig fraction
NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia.
Demonstrated to react with Rat. Predicted to react with Mouse based on 100% sequence homology. Unconjugated Anti-NeuN has been shown to work with multiple species including Human, Mouse, Ferret, Chicken, and Avian.

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