discontinued

AM05303PU-N Neuregulin 1 antibody

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Quick Overview

Mouse anti Human, Mouse, Rat Neuregulin 1 7D5

Product Description for Neuregulin 1

Mouse anti Human, Mouse, Rat Neuregulin 1 7D5.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for Neuregulin 1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, GGF, Glial growth factor, HGL, HRG, HRGA, Heregulin, NDF, NRG1, Neu differentiation factor, Pro-neuregulin-1 membrane-bound isoform, SMDF, Sensory and motor neuron-derived factor
Presentation Purified
Reactivity Hu, Ms, Rt
Applications C, IP, P, WB
Clonality Monoclonal
Clone 7D5
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q02297
Immunogen:
Hybridoma produced by the fusion of spelocytes from BALB/c mice immunized with recombinant extracellular domain of rat NDF protein coupled to KLH and mouse myeloma NSO cells.
GeneID:
3084
Isotype control AM03096PU-N
Application Western Blot (1-5 µg/ml).
Immunoprecipitation (2 µg/mg of protein lysate).
Immunohistochemistry on Frozen and Formalin/Paraffin Sections (1/20-1/40).
Positive Control: HRGa1, HRGb1 recombinant protein or MDA-MB-231 cells; prostate carcinoma.
Background HRG/NDF exists in several isoforms, which are classified in two groups alpha and beta.
Concentration 1 mg/ml
General Readings
  1. Graus-Porta D, Beerli RR, Hynes NE. Single-chain antibody-mediated intracellular retention of ErbB-2 impairs Neu differentiation factor and epidermal growth factor signaling. Mol Cell Biol. 1995 Mar;15(3):1182-91. PubMed PMID: 7532277. (Free PMC Article available)
  2. Devarajan K, et al. (1996) ErbB-2 is a common auxiliary subunit of NDF- and EGF-receptors: implications for breast cancer. EMBO. J. 15 : 254-264.
  3. Marte BM, Graus-Porta D, Jeschke M, Fabbro D, Hynes NE, Taverna D. NDF/heregulin activates MAP kinase and p70/p85 S6 kinase during proliferation or differentiation of mammary epithelial cells. Oncogene. 1995 Jan 5;10(1):167-75. PubMed PMID: 7824269.
  4. Beerli RR, Graus-Porta D, Woods-Cook K, Chen X, Yarden Y, Hynes NE. Neu differentiation factor activation of ErbB-3 and ErbB-4 is cell specific and displays a differential requirement for ErbB-2. Mol Cell Biol. 1995 Dec;15(12):6496-505. PubMed PMID: 8524214. (Free PMC Article available)
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
PBS containing 0.08% Sodium Azide as preservative.
Purity:
Purified
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
Recognizes a glycoprotein of 44kDa, identified as heregulin (HRG) or neu differentiation factor (NDF) which binds to c-erbB-3 and c-erbB-4 receptors with low and high affinities, respectively.
This antibody is directed against the Extracellular domain of NDF/HRG and is highly specific. It shows no cross-reaction with EGF and reacts with both alpha- and beta-isoforms of NDF/HRG, suggesting that its epitope is outside the EGF-domain.
This antibody does not inhibit the binding of HRG to ErbB receptors and is excellent for multiple applications.
Species:
Human, Rat and Mouse.
Gene ID 3084

Accessory Products

Proteins and/or Positive Controls

Proteins for Neuregulin 1 (13 products)

Catalog No. Species Pres. Purity   Source  

Neuregulin 1 (transcript variant GGF2)

Neuregulin 1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €139.00
  OriGene Technologies, Inc.

Neuregulin 1 (beta-1 Isoform)

Neuregulin 1 Human Purified > 98 % > 98 % by SDS-PAGE gel and HPLC analyses E. coli
  OriGene Technologies GmbH

Neuregulin 1 (beta-1 Isoform)

Neuregulin 1 Human Purified > 98 % > 98 % by SDS-PAGE gel and HPLC analyses E. coli
  OriGene Technologies GmbH

Neuregulin 1

Neuregulin 1 Human Purified
  Abnova Taiwan Corp.

Neuregulin 1

Neuregulin 1 Human Purified
  Abnova Taiwan Corp.

Neuregulin 1

Neuregulin 1 Human
  Abnova Taiwan Corp.

Neuregulin 1

Neuregulin 1 Human
  Abnova Taiwan Corp.

Neuregulin 1

Neuregulin 1 Human
  Abnova Taiwan Corp.

Neuregulin 1

 Heregulin-B2 structure and sequence Human Purified > 95 % E. coli
10 µg / €310.00
  Neuromics Antibodies

Neuregulin 1

 Heregulin-B2 structure and sequence Human Purified > 95 % E. coli
50 µg / €440.00
  Neuromics Antibodies

Neuregulin 1

Functional Assay: Neuregulin 1 Protein [NBC1-21353] - The bioactivity was determined by dose-dependent cell proliferation assay for MCF-7 cells. ED50 for this NovActive™ NRG1 LOT lies in the range of 0.9-1.3 ng/ml. Affinity purified
  Novus Biologicals Inc.

Neuregulin 1

Neuregulin 1 Affinity purified
  Novus Biologicals Inc.

Neuregulin 1

Neuregulin 1 Affinity purified
  Novus Biologicals Inc.

Positive controls for Neuregulin 1 (4 products)

Catalog No. Species Pres. Purity   Source  

NRG1 293T Cell Transient Overexpression Lysate(Denatured)

NRG1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

NRG1 293T Cell Transient Overexpression Lysate(Denatured)

NRG1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

NRG1 Lysate

Western Blot: NRG1 Lysate [NBL1-13790] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for NRG1
  Novus Biologicals Inc.

NRG1 overexpression lysate

NRG1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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