AP00573PU-N NF-kB p50 antibody

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Rabbit anti Human NF-kB p50

Product Description for NF-kB p50

Rabbit anti Human NF-kB p50.
Presentation: Purified
Product is tested for Paraffin Sections.

Properties for NF-kB p50

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms DP3090, NFkB, NFkappaB
Presentation Purified
Reactivity Hu
Applications P
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

A synthetic peptide corresponding to human NF kappa B p50 protein.
AA Sequence:
Mol. Wt. of Antigen: 50 kDa
Application Immunohistology (Formalin/paraffin): use 10-20 µg/ml for 30 Mins at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: Testis Leyding cells
Background NF kappa B is a heterodimer consisting of a 50kDa DNA binding subunit and a 65kDa transactivating subunit (RelA). p50 subunit of NFkB has an isoform and both the proteins are derived from larger precursor proteins of 105 and 100kDa respectively. The p50/p65 heterodime is present in the cytoplasm in inactive form bound to its inhibitor, IκB. Upon stimulation of cells with stimuli like LPS, Interleukin-1, IkB gets phosphorylated and degraded by proteosomes releasing the activated NFkB which goes to the nucleus inducing gene expression. NFkB was originally identified as transcriptional activator for kappa light cahain gene in B cells.
Concentration 1.0 mg/ml
General Readings
  1. Ghosh S, et al. (1998) Annu. Rev. Immunol. 16: 225-260.
  2. Schmid R M, et al. (1991) Nature 352: 733-736.
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.
Liquid IgG
This antibody detects NF kappa B.
Cellular Localization: Cytoplasmic and Nuclear

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