SP6383P NF-kB p50 antibody

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Rabbit anti Human, Rabbit, Rat NF-kB p50


Product Description for NF-kB p50

Rabbit anti Human, Rabbit, Rat NF-kB p50.
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for NF-kB p50

Product Category Primary Antibodies
Quantity 50 µg
Synonyms NFkB, NFkappaB
Presentation Aff - Purified
Reactivity Hu, Rb, Rt
Applications IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer AnaSpec

Datasheet Extract

A synthetic peptide sequence corresponding to internal domain of human NFkB p50.

Format: This antibody is supplied as liquid epitope affinity purified immunglobulin fraction in 10 mM Phosphate buffered saline pH 7.4 with BSA as stabilizer and 0.02% Proclin300 as preservative.
Application ELISA (0.1 - 1.0 µg/ml), Western blot (0.5 - 2µg/ml), Immunoprecipitation (3.0 - 5.0 µg/extract from 107 cells) and Immunohistochemistry (1/100 or 10 µg/ml for 10 min at room temperature; formalin-fixed tissues requires boiling of tissue sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min). Use prostate or placenta tissue as positive control. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
General Readings
  1. Mabuchi S. et al. Biol. Chem., 10,1074 (2004)
  2. Romashkova JA and Makarov SS. Nature 401, 86 (1999)
  3. Nolan GP. Cell 64, 961(1991)
  4. Lentsch A. Immunol Ther Exp 48, 59 (2000)
  5. Hatada EN. et al. Curr Opin Immunol 12, 52 (2000).
  6. Magnan
Storage Store the antibody undiluted at 4-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Aff - Purified
Nuclear factor kappa-B (NFkB) is a transcription factor regulating numerous genes. It is implicated in a variety of cellular events such as in immune response, growth, cellular survival, and development of several pathological states. NF-kB family is composed of homo- and heterodimers resulting from the combinatorial association of five polypeptides: p50, p52, p65, cRel, and RelB, forming mostly NFkB1p50/RelAp65 heterodimers. Different combinations of NF-kB/Rel proteins regulate the transcription of different genes. The 50KDa is a DNAbinding subunit. NFkB normally resides in the cytoplasm in an inactive form as a complex with IkB kinase. Phosphorylation of IkB promotes its degradation, allowing NFkB to translocate to the nucleus and induce target genes. The transcription factor NFkB is a primary regulator of a wide variety of proinflammatory mediators, inappropriate activation of NFkB is associated with a number of inflammatory diseases. NFkB plays a role in PDGF signaling, being a target of AKT in anti-apoptotic signaling.
This antibodydetects human, rat and rabbit NFkBp50. It does not react with mouse.

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