AP02651PU-S NFKBIA / IKBA antibody

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50 µg / €240.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse NFKBIA / IKBA


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  • AP02651PU-S
  • AP02651PU-S

Product Description for NFKBIA / IKBA

Rabbit anti Human, Mouse NFKBIA / IKBA.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for NFKBIA / IKBA

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI
Presentation Aff - Purified
Reactivity Hu, Ms
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Synthetic non-phosphopeptide derived from human IkappaB-alpha around the phosphorylation site of serine 32/36 (H-D-S-G-L-D-S-M-K).
Application Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry on paraffin sections: 1/50 -1/100.
Immunofluorescence: 1/100 - 1/200.
Background Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IkappaB alpha and IkappaB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkappaB and block nuclear import by masking the nuclear localisation sequences of NFkappaB. The activation of NFkappaB involves the inducible phosphorylation and subsequent degradation of IkappaB. Immunoblotting easily detects the hyperphosphorylated forms of IkappaB alpha, but not phosphorylated IkappaB beta. Interestingly, IkappaB alpha and IkappaB beta mediate different NFkappaB responses. IkappaB alpha appears to control more transient activation of NFkappaB in response to an inducer, while IkappaB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkappaB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkappaB is confirmed by immunoblotting.
Concentration 1.0 mg/ml
General Readings
  1. Mattioli I, et al. (2004)J Immunol; 172(10): 6336-44.
  2. Courtois G, et al. (2003)J Clin Invest; 112(7): 1108-15.
  3. Nair A, et al. (2003) Oncogene; 22(1): 50-8.
  4. Fan C, et al. (2002)J Cell Sci; 115(Pt 24): 4843-53.
  5. Schubert SY, et al. (2002)FASEB J; 16(14): 1931-3.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Immunoaffinity chromatography
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Liquid purified Ig fraction
Aff - Purified
IkappaB-alpha antibody detects endogenous levels of total IkappaB-alpha protein.
Human, Mouse and Rat.
Gene ID 4792

Accessory Products

Proteins and/or Positive Controls

Proteins for NFKBIA / IKBA (5 products)

Catalog No. Species Pres. Purity   Source  


NFKBIA / IKBA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

NFKBIA / IKBA (1-317, His-tag)

NFKBIA / IKBA Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €940.00
  OriGene Technologies GmbH

NFKBIA / IKBA (1-317, His-tag)

NFKBIA / IKBA Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €370.00
  OriGene Technologies GmbH


NFKBIA / IKBA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.


NFKBIA / IKBA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.
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