AP09460PU-N NFkBp65 pThr435 antibody

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Rabbit anti Human NFkBp65

Product Description for NFkBp65

Rabbit anti Human NFkBp65.
Properties: pThr435
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for NFkBp65

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms NF-kB p65, NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, Transcription factor p65
Presentation Aff - Purified
Reactivity Hu
Applications ICC/IF
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

The antiserum was produced against synthesized phosphopeptide derived from Human Rel around the phosphorylation site of Serine 503 (T-S-SP-D-S).
Property pThr435
Application Immunofluorescence: 1/100~1/200.
Background c-Rel is the cellular cognate of v-Rel, the avian reticuloendotheliosis virus strain T transforming gene. v-Rel encodes a phosphoprotein that is located in the cytoplasm of transformed spleen cells and in the nucleus of non-transformed fibroblasts, in contrast to the c-Rel protein, which is cytoplasmic. c-Rel has been shown to represent a constituent of the kappaB site binding transcription factor NFkappaB, which plays a crucial role in the expression of immunoglobulin kappa light chain gene. In contrast to c-Rel, v-Rel is truncated in its C-terminal transactivation domain and does not appear to function as a transcriptional transactivator. It has thus been postulated that v-Rel may interfere with the normal transcription of NFkappaB regulated genes and thus cause transformation by a mechanism analogous to v-ErbA, which binds to the thyroid hormoneresponsive region in certain erythroid genes needed for differentiation, but cannot be activated by thyroid hormone.
Concentration 1.0 mg/ml
General Readings
  1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
  2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
  3. Haskill, S. et al. (1991) Cell 65, 1281-9.
  4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4 with 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Liquid purified Ig fraction.
Aff - Purified
This antibody detects endogenous levels of Rel only when phosphorylated at Serine 503.

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