discontinued

AP09460PU-N NFkBp65 pThr435 antibody

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0.1 mg / €310.00

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Rabbit anti Human NFkBp65

Product Description for NFkBp65

Rabbit anti Human NFkBp65.
Properties: pThr435
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for NFkBp65

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms NF-kB p65, NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, Transcription factor p65
Presentation Aff - Purified
Reactivity Hu
Applications ICC/IF
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from Human Rel around the phosphorylation site of Serine 503 (T-S-SP-D-S).
Property pThr435
Application Immunofluorescence: 1/100~1/200.
Background c-Rel is the cellular cognate of v-Rel, the avian reticuloendotheliosis virus strain T transforming gene. v-Rel encodes a phosphoprotein that is located in the cytoplasm of transformed spleen cells and in the nucleus of non-transformed fibroblasts, in contrast to the c-Rel protein, which is cytoplasmic. c-Rel has been shown to represent a constituent of the kappaB site binding transcription factor NFkappaB, which plays a crucial role in the expression of immunoglobulin kappa light chain gene. In contrast to c-Rel, v-Rel is truncated in its C-terminal transactivation domain and does not appear to function as a transcriptional transactivator. It has thus been postulated that v-Rel may interfere with the normal transcription of NFkappaB regulated genes and thus cause transformation by a mechanism analogous to v-ErbA, which binds to the thyroid hormoneresponsive region in certain erythroid genes needed for differentiation, but cannot be activated by thyroid hormone.
Concentration 1.0 mg/ml
General Readings
  1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
  2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
  3. Haskill, S. et al. (1991) Cell 65, 1281-9.
  4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4 with 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State:
Liquid purified Ig fraction.
Aff - Purified
Specificity
Specificity:
This antibody detects endogenous levels of Rel only when phosphorylated at Serine 503.
Species:
Human.

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