NB100-143 Nibrin antibody

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Rabbit anti Human, Monkey, Mouse Nibrin


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  • NB100-143

Product Description for Nibrin

Rabbit anti Human, Monkey, Mouse Nibrin.
Presentation: Serum
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Nibrin

Product Category Primary Antibodies
Target Category
Quantity 50 µl
Synonyms Cell cycle regulatory protein p95, NBN, NBS, NBS1, Nijmegen breakage syndrome protein 1, P95
Presentation Serum
Reactivity Hu, Mky, Ms
Applications ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Swiss Prot Num:
Human NBS1 protein (betwqeen residues 1-705). [Swiss-Prot# O60934]
Add. information Research Areas: DNA Repair, Cancer
Application Immunocytochemistry, immunofluorescence, immunoprecipitation, Western Blot, By Western blot, this antibody recognizes a band at 95 kDa, representing NBS1. By immunofluorescence, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. Immunoprecipitation tests have been done with 3-4 X 106 cells. , immunofluorescence 1:200, immunoprecipitation 3 ul, Western Blot 1:1000, Immunocytochemistry 1:50 to 1:200
Background The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
General Readings
  1. Wu X, Petrini JH, Heine WF, Weaver DT, Livingston DM, Chen J. Independence of R/M/N focus formation and the presence of intact BRCA1. Science. 2000 Jul 7;289(5476):11. PubMed PMID: 10928918.
  2. Gatei M, Young D, Cerosaletti KM, Desai-Mehta A, Spring K, Kozlov S, et al. ATM-dependent phosphorylation of nibrin in response to radiation exposure. Nat Genet. 2000 May;25(1):115-9. PubMed PMID: 10802669.
  3. Stracker TH, Carson CT, Weitzman MD. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature. 2002 Jul 18;418(6895):348-52. PubMed PMID: 12124628.
  4. Huang J, Dynan WS. Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction. Nucleic Acids Res. 2002 Feb 1;30(3):667-74. PubMed PMID: 11809878. (Free PMC Article available, 5 images available)
  5. Giannini G, Ristori E, Cerignoli F, Rinaldi C, Zani M, Viel A, et al. Human MRE11 is inactivated in mismatch repair-deficient cancers. EMBO Rep. 2002 Mar;3(3):248-54. Epub 2002 Feb 15. PubMed PMID: 11850399. (Free PMC Article available, 4 images available)
  6. Lukas C, Falck J, Bartkova J, Bartek J, Lukas J. Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage. Nat Cell Biol. 2003 Mar;5(3):255-60. PubMed PMID: 12598907.
  7. Brown KD, Rathi A, Kamath R, Beardsley DI, Zhan Q, Mannino JL, et al. The mismatch repair system is required for S-phase checkpoint activation. Nat Genet. 2003 Jan;33(1):80-4. Epub 2002 Nov 25. PubMed PMID: 12447371.
  8. Gueven N, Becherel OJ, Birrell G, Chen P, DelSal G, Carney JP, et al. Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype. Cancer Res. 2006 Mar 15;66(6):2907-12. PubMed PMID: 16540636.
  9. Verdun RE, Crabbe L, Haggblom C, Karlseder J. Functional human telomeres are recognized as DNA damage in G2 of the cell cycle. Mol Cell. 2005 Nov 23;20(4):551-61. PubMed PMID: 16307919.
  10. Compton SA, Choi JH, Cesare AJ, Ozgür S, Griffith JD. Xrcc3 and Nbs1 are required for the production of extrachromosomal telomeric circles in human alternative lengthening of telomere cells. Cancer Res. 2007 Feb 15;67(4):1513-9. PubMed PMID: 17308089.
  11. Dote H, Burgan WE, Camphausen K, Tofilon PJ. Inhibition of hsp90 compromises the DNA damage response to radiation. Cancer Res. 2006 Sep 15;66(18):9211-20. PubMed PMID: 16982765.
  12. McCabe N, Turner NC, Lord CJ, Kluzek K, Bialkowska A, Swift S, et al. Deficiency in the repair of DNA damage by homologous recombination and sensitivity to poly(ADP-ribose) polymerase inhibition. Cancer Res. 2006 Aug 15;66(16):8109-15. PubMed PMID: 16912188.
  13. Mirzoeva OK, Kawaguchi T, Pieper RO. The Mre11/Rad50/Nbs1 complex interacts with the mismatch repair system and contributes to temozolomide-induced G2 arrest and cytotoxicity. Mol Cancer Ther. 2006 Nov;5(11):2757-66. PubMed PMID: 17121922.
  14. Takemura H, Rao VA, Sordet O, Furuta T, Miao ZH, Meng L, et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem. 2006 Oct 13;281(41):30814-23. Epub 2006 Aug 10. PubMed PMID: 16905549.
Storage Store at 4C. Do not freeze.
Whole antisera
Buffer System:
0.02% Sodium Azide
Whole antisera
This is specific for human NBS1.
Human, Mouse (-)
Gene ID 4683

Accessory Products

Proteins and/or Positive Controls

Proteins for Nibrin (3 products)

Catalog No. Species Pres. Purity   Source  


Nibrin Human
  Abnova Taiwan Corp.


Nibrin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.


Nibrin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Nibrin (1 products)

Catalog No. Species Pres. Purity   Source  

NBS1 Lysate

Western Blot: NBS1 Lysate [NBL1-13497] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for NBN
  Novus Biologicals Inc.
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