AM00294PU-N Nidogen-1 / Entactin antibody

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Rat anti Guinea Pig, Mouse Nidogen-1 / Entactin ELM1


Product Description for Nidogen-1 / Entactin

Rat anti Guinea Pig, Mouse Nidogen-1 / Entactin ELM1.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Nidogen-1 / Entactin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Ent, NID-1, Nid1
Presentation Purified
Reactivity GP, Ms
Applications C, ICC/IF, P
Clonality Monoclonal
Clone ELM1
Host Rat
Isotype IgG2a
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Murine EHS laminin preparation.
Mol. Wt. of Antigen: 150 kDa (intact); 135 kDa, 110 kDa, 100 kDa, and 80 kDa (proteolytic fragments).
Isotype control SM15P, SM15PX
Application Immunofluorescence.
Immunohistology (Acetone-fixed frozen and Alcohol-fixed, paraffin-embedded) (Use Ab at 2µg/ml for 30 min at RT).
Positive Control: Mouse kidney.
Background Entactin/Nidogen is a ubiquitous multidomain basement membrane protein. Hirschsprung's disease (congenital colonic aganglionosis) is associated with abnormalities in the distributions and amounts of basement membrane and other extracellular matrix components in the human gut. Corneas of diabetic patients have abnormal healing and epithelial adhesion, which may be due to alterations of the corneal extracellular matrix and basement membrane. Modified expression, production, and deposition of basement membrane components in high metastatic malignant melanoma cells could be involved in their altered interactions with the extracellular matrix.
Concentration 0.2 mg/ml
General Readings
  1. Horiguchi et. al., Arch Dermatol Res, 1989; 281:427-432.
  2. Ljubimov AV et. al., Exp Cell Res, 1986; 165:530-540.
Storage Store the antibody at 2 - 8 °C for one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.
Liquid ascites fluid.
This antibody detects Entactin / Nidogen.
It does not cross-react with other basement membrane components or fibronectin.
Cellular Localization: Basement membrane.
Guinea pig, Mouse. Does not react with Human.

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