discontinued

NB100-64245 NK-1.1 antibody

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0.1 mg / €370.00

Quick Overview

Mouse anti Mouse NK-1.1 PK136

NB100-64245

Product Description for NK-1.1

Mouse anti Mouse NK-1.1 PK136.
Presentation: Biotin
Product is tested for Flow Cytometry.

Properties for NK-1.1

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Biotin
Reactivity Ms
Applications F
Clonality Monoclonal
Clone PK136
Host Mouse
Isotype IgG2a
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Spleen and bone marrow cells from CE mice
Application flow cytometry / FACS analysis, Flow cytometry / FACS analysis, flow cytometry / FACS analysis
Background NK1.1, a member of the NKRPI family of cell surface receptors, is a type II integral membrane protein with a C type lectin domain. It is expressed as a disulfide linked homodimer on all NK cells, as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57/BL6, FVB/N, NZB; but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, SJL, 129). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This antibody has been shown to be useful in studies of "in vitro" complement dependent cytotoxicity assays, "in vitro" redirected lysis and "in vivo" and "in vitro" activation of NK1.1 cells.
Concentration 0.1 mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
Biotin
Purification:
protein A purified
Buffer System:
0.09% Sodium Azide
Purity:
protein A purified
Specificity
Specificity:
NB100-64245 (clone PK136) recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc. Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. NB100-64245 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.
Species:
Human (-), Mouse, Rat (-)

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