SM2091PS NKG2A/C/E antibody

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Rat anti Mouse NKG2A/C/E 20d5


Product Description for NKG2A/C/E

Rat anti Mouse NKG2A/C/E 20d5.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for NKG2A/C/E

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CD159a, CD159c, CD159e, NKG2 lectin-like family
Presentation Purified
Reactivity Ms
Applications F
Clonality Monoclonal
Clone 20d5
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

CHO transfected cells expressing the B6 allele of NKG2A. Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Isotype control SM15P, SM15PX
Application Flow Cytometry (1/25-1/100): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Background In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse.
Concentration 1.0 mg/ml
General Readings
  1. Vance RE, Jamieson AM, Raulet DH. Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells. J Exp Med. 1999 Dec 20;190(12):1801-12. PubMed PMID: 10601355. (Free PMC Article available, 7 images available)
  2. Vance RE, Jamieson AM, Cado D, Raulet DH. Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation. Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):868-73. Epub 2002 Jan 8. PubMed PMID: 11782535. (Free PMC Article available, 5 images available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G.
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
Liquid purified IgG fraction.
This antibody recognises NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family.
Clone 20d5 is reported to block ligand binding to the receptor. We recommend the use of SM2091LE for this purpose.

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