AP01490PU-N NLE1 antibody

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0.1 mg / €350.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat NLE1

Product Description for NLE1

Rabbit anti Human, Mouse, Rat NLE1.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for NLE1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms HUSSY-07, Notchless protein homolog 1
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~ 48 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9NVX2
GeneID:
54475
Application Western Blot: 1/500-1/1000.
Background The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway.In plants, NLE1 is crucial for normal cellular growth and development. Underexpression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.
Concentration 1.0 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
NLE1 antibody detects endogenous levels of NLE1 protein.(region surrounding Tyr333)
Species:
Human, Mouse, Rat.
Gene ID 54475

Accessory Products

Proteins and/or Positive Controls

Proteins for NLE1 (1 products)

Catalog No. Species Pres. Purity   Source  

NLE1 (transcript variant 1)

NLE1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Positive controls for NLE1 (2 products)

Catalog No. Species Pres. Purity   Source  

NLE1 Lysate

Western Blot: NLE1 Lysate [NBL1-13664] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for NLE1
  Novus Biologicals Inc.

NLE1 overexpression lysate

NLE1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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