NB100-2071 Nonspecific Cytotoxic Cells antibody

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Mouse anti Bovine, Canine, Equine, Fish, Porcine, Rat Nonspecific Cytotoxic Cells 5C.6


Product Description for Nonspecific Cytotoxic Cells

Mouse anti Bovine, Canine, Equine, Fish, Porcine, Rat Nonspecific Cytotoxic Cells 5C.6.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry, Western blot / Immunoblot.

Properties for Nonspecific Cytotoxic Cells

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Bov, Can, Eq, Fi, Por, Rt
Applications E, F, WB
Clonality Monoclonal
Clone 5C.6
Host Mouse
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Purified NCC cells from catfish.
Isotype control SM13P
Application ELISA, flow cytometry / FACS analysis, immunoprecipitation, Western Blot, Western Blot, ELISA, FACS, Immunoprecipitation Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. This antibody as been successfully used in cytotoxic inhibition studies with NCC/NK/LAK cells, panning, and ELISA procedures. By Western blot, this antibody detects a 32 kDa protein representing nonspecific cytotoxic cell receptor protein. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation., flow cytometry / FACS analysis, ELISA, immunoprecipitation, Western Blot 10 ug/ml,
Background Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
General Readings This product has been used in:Jaso-Friedmann L et al. NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells. Mol Immunol 34:955-65 (1997). PubMed PMID: 9464530
General / background references:Viveiros MM & Antczak DF Characterization of equine natural killer and IL-2 stimulated lymphokine activated killer cell populations. Dev Comp Immunol 23:521-32 (1999). PubMed PMID: 10512462
Raymond C & Wilkie BN Natural killer cell frequency and function in pigs selectively bred for high or low antibody and cell-mediated immune response: response to vaccination with modified live transmissible gastroenteritis virus. Nat Immun 16:18-26 (1998). PubMed PMID: 9789121
Evans DL et al. Nonspecific cytotoxic cell receptor protein-1: a novel (predicted) type III membrane receptor on the teleost equivalent of natural killer cells recognizes conventional antigen. Cell Immunol 187:19-26 (1998). PubMed PMID: 9681999
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
IgM purified
Buffer System:
0.05% Sodium Azide
IgM purified
Nonspecific Cytotoxic Cell (5C.6)
Bovine, Canine, Equine, Fish, Porcine, Rat

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