SM5086 NOS (pan NOS) antibody

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Mouse anti Bovine, Mouse, Rat NOS NOS-3F7-B11 B5


Product Description for NOS

Mouse anti Bovine, Mouse, Rat NOS NOS-3F7-B11 B5.
Properties: (pan NOS)
Presentation: Ascites
Product is tested for Frozen Sections, Western blot / Immunoblot.

Properties for NOS

Product Category Primary Antibodies
Quantity 0.2 ml
Synonyms NOS1, NOS2, NOS3, bNOS and eNOS, iNOS
Presentation Ascites
Reactivity Bov, Ms, Rt
Applications C, WB
Clonality Monoclonal
Clone NOS-3F7-B11 B5
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Purified bovine bNOS.
Property pan NOS
Application Immunohistochemistry on frozen sections: 1/100, staining of NOS with SM5086 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.
Western Blot: 1/250, detects an ~130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an ~155 kDa band in tissues expressing bNOS and an ~140 kDa band in tissues expressing eNOS.
Background Nitric Oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells including vasorelaxation, neurotransmission and cytotoxicity. NO is produced by a Nitric Oxide Synthase (NOS) family of enzymes which oxidizes the amino acid L-arginine to form L-citrulline and NO. Two constitutive forms, brain NOS (bNOS) & endothelial cell NOS (eNOS), and one inducible form (iNOS), have been cloned showing regions for binding of calmodulin, NADPH, FAD, and FMN.
iNOS has been found in a variety of tissues including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. It is induced after exposure to cytokines such as interferon-* (IFN), tumor necrosis factor, interleukin-1, interleukin-2, and lipopolysaccarides (LPS). After cytokine induction, iNOS has a delayed response followed by high levels of NO produced over a long period of time.
eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells. eNOS is capable of synthesizing NO in vascular endothelial cells where it appears to play an important role in the control of vasotension and platelet aggregation.
General Readings
  1. Am. J. Physiol., 267: L704-L711, 1994.
  2. Am. J. Physiol., 267: L667-L678, 1994.
  3. FASEB Journal, 6: 3051-3064, 1992.
  4. J. Biol. Chem., Vol 278: 41798 - 41803, Oct 2003
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS containing 0.05% sodium azide as preservative.
Liquid diluted ascites.
This antibody detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS) in bovine, mouse and rat tissues.

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