BM2231P Nuclear Antigen (Membrane) antibody

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0.1 mg / €310.00
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Mouse anti Human Nuclear Antigen (Membrane) AE-5


Product Description for Nuclear Antigen (Membrane)

Mouse anti Human Nuclear Antigen (Membrane) AE-5.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections.

Properties for Nuclear Antigen (Membrane)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Nuclear membrane marker
Presentation Purified
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone AE-5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Nuclei of myeloid leukemia biopsy cells.
Isolated splenocytes were fused with mouse myeloma cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow Cytometry. 
Immunohistochemistry on Frozen and Paraffin Sections: AE-5 produces a ring staining pattern around the nucleus of normal and malignant cells and can be used for paraformaldehyde fixed or frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Recommended Positive Control: Tonsil tissue.
Background The nuclear envelope is riddled with nuclear pores that allow specific materials to pass in and out of the nucleus. Attached to the nuclear envelope is the endoplasmic reticulum.
Concentration 0.1 mg/ml
General Readings This clone has not been published but similarly generated and characterized clones are described in:
1. Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using
monoclonal antibodies. In: Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Buffer System:
0.05% Sodium Azide
Liquid purified IgG fraction
Species Reactivity
Species reactivity (tested):
This antibody clone AE-5 recognizes an antigen associated with the nuclear membrane expressed in Human cells. 
This Monoclonal antibody clone AE-5 can also be used as a Marker of the nuclear membrane in subcellular fractions.

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