DM3156 Nuclear Antigen (Nucleoli) antibody

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Mouse anti Human Nuclear Antigen (Nucleoli) B462


Product Description for Nuclear Antigen (Nucleoli)

Mouse anti Human Nuclear Antigen (Nucleoli) B462.
Presentation: Supernatant
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections.

Properties for Nuclear Antigen (Nucleoli)

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Supernatant
Reactivity Hu
Applications C, F, P
Clonality Monoclonal
Clone B462
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Myeloid leukemia cell nuclei.

Format: This antibody is supplied as liquid, purified immunoglobulin fraction in 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% Sodium Azide, pH 7.5.
Application Flow cytometry (1:150-1:300). Immunohistochemistry on frozen and paraffin embedded sections (1/50- 1/100; Recommended positive control: Tonsil). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.3 mg/ml
General Readings
  1. Epstein, A.L. et al, Progress on nonhistone protein
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody reacts with nucleolar antigen which is expressed in the nucleoli of human cells.

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