discontinued

BM645S Nuclear Antigen (NuMa related) antibody

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0.5 ml / €170.00

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Mouse anti Human Nuclear Antigen (NuMa related) A73-B/D12

BM645S

Product Description for Nuclear Antigen (NuMa related)

Mouse anti Human Nuclear Antigen (NuMa related) A73-B/D12.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Nuclear Antigen (NuMa related)

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Supernatant
Reactivity Hu
Applications C, ICC/IF, P
Clonality Monoclonal
Clone A73-B/D12
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Live Ls 174T cells (colon carcinoma).
Application Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/100. For paraffin sections high temp. antigen demasker, 10mM citrate, pH 6.0, is required. This antibody can be used on frozen and formalin tissue sections. Formalin-fixed paraffin-embdded tissue sections require, boilng the tisssue in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling to Rt for 10-20 mins. This step is done prior to antibody. The antibody may be used at a dilution of 1:50-1:100 with AutoProbe III (Cat. No. 08-803). Western Blot: 1/100 - 1/300. Recommended positive control: Tonsil, Thymus, Spleen. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.3 mg/ml
General Readings
  1. Compton DA, Szilak I, Cleveland DW. Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis. J Cell Biol. 1992 Mar;116(6):1395-408. PubMed PMID: 1541636. (Free PMC Article available)
  2. Butschak G, Harborth J, Osborn M, Karsten U. New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells. Acta Histochem. 1995 Jan;97(1):19-31. PubMed PMID: 7771183.
  3. Gueth-Hallonet, C. etal, Cleavage of the nuclear matrix protein NuMA durin
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Format
State:
Supernatant
Specificity
Specificity:
Reacts with an intracellular 228 kD protein found in the nucleus during interphase.

Mol. Wt. of Antigen: 228 kD

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