AP20131PU-N Nuclear Pore Complex Marker (pan) antibody

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0.5 ml / €310.00
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Mouse anti Human, Mouse, Rat, Yeast Nuclear Pore Complex Marker 39C7


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  • AP20131PU-N

Product Description for Nuclear Pore Complex Marker

Mouse anti Human, Mouse, Rat, Yeast Nuclear Pore Complex Marker 39C7.
Properties: (pan)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for Nuclear Pore Complex Marker

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms 107 kDa nucleoporin, NPC, NUP107, NUP84, Nuclear Pore Complex Proteins, Nup133, Nup62
Presentation Purified
Reactivity Hu, Ms, Rt, Ye
Applications ICC/IF
Clonality Monoclonal
Clone 39C7
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Yeast nuclear preparations and screening the resullting hybridomas by Immunofluorescence on Yeast cells.
Property pan
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Immunofluorescence: 1/100-1/500 (Yeast cells) and 1/50-1/100 (Mammalian cells). 
Note: This antibody does not work well on Western blots so we are currently unsure of the exact identity of the protein to which it binds.
Background The Nuclear pore complex (NPC) has a molecular mass of ~125 KDa in vertebrates and contains about 50 or more different proteins . The NPC spans the dual membrane of the the nuclear envelope (NE) and acts as a gateway for macromolecular traffic between the cytoplasm and the nucleus. The basic framework of the NPC consists of a central core with a ring-spoke structure exhibiting 8 fold radial symmetry. From this central ring 50 to 100 nm fibrils extend into the nucleoplasm and the cytoplasm. The NPC is in turn anchored in the NE by the nuclear lamina, a meshwork of lamins and lamin-associated proteins that forms a 15 nm thick fibrous structure between the inner nuclear membrane and peripheral chromatin. A number of proteins called nucleoporins have been localised to discrete regions of the NPC and are often used as markers for this compartment, e.g. Nup153. Approximately half of the nucleoporins (or Nups) contain a phenyalanine-glycine repeat motif (FG repeat), which may be diagnostic for proteins playing a role in nuclear transport.
Concentration 0.25 mg/ml
Product Citations
Purchased from Acris:
  1. Vietri M, Schink KO, Campsteijn C, Wegner CS, Schultz SW, Christ L, et al. Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing. Nature. 2015 Jun 11;522(7555):231-5. doi: 10.1038/nature14408. Epub 2015 Jun 3. PubMed PMID: 26040712.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G Chromatography
10 mM Sodium Azide
Liquid Sterile-filtered Cell Culture fluid from an Integra CL-350 bio-chamber
This Monoclonal antibody is a Panspecific Nuclear Pore Complex Marker.
Clone 39C7 was one of a series of clones which strongly and specifically labelled the Nuclear Pore Complex.
When this antibody was tested on cells from other species, including Rat, Mouse and Human cells, it has invariably strongly stained nuclear pore complexes, so it appears to be an excellent and panspecific marker for these important structures.
Human, Mouse, Rat and Yeast.
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