NB600-1068 Nuclear Pore-O-Linked Glycoprotein antibody

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Mouse anti Human, Mammalia, Mouse, Rat, Xenopus, Yeast Nuclear Pore-O-Linked Glycoprotein RL1


Product Description for Nuclear Pore-O-Linked Glycoprotein

Mouse anti Human, Mammalia, Mouse, Rat, Xenopus, Yeast Nuclear Pore-O-Linked Glycoprotein RL1.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Nuclear Pore-O-Linked Glycoprotein

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms Nuclear Pore Marker
Presentation Purified
Reactivity Hu, Mam, Ms, Rt, Xen, Ye
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone RL1
Host Mouse
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Isotype control SM13P
Application immunofluorescence, immunoprecipitation, Western Blot, Immunofluorescence, Western Blot, and Immunoprecipitation This antibody detects up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa. Immunofluorescence staining of NPC O-linked glycoproteins with this antibody results in exclusive labeling of the NPC proteins on a wide variety of mammalian cells as well as S. cerevisiae and Xenopus. Labeling occurs exclusively at the NPC with most of the labeling at the cytoplasmic and nucleoplasmic margins.This antibody, if microinjected inhibits both protein import and RNA export in Xenopus oocytes., immunofluorescence 1:100, immunoprecipitation Assay dependent, Western Blot 1:1000
Background Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC
General Readings This product has been used in:Gorsch LC et al. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129:939-55 (1995). PubMed PMID: 7744966
Guan T et al. Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol Biol Cell 6:1591-603 (1995). PubMed PMID: 8589458
Byrd DA et al. Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex. J Cell Biol 127:1515-26 (1994). PubMed PMID: 7798308
Greber UF & Gerace L Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein. J Cell Biol 116:15-30 (1992). PubMed PMID: 1370490
Sterne-Marr R et al. O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import. J Cell Biol 116:271-80 (1992). PubMed PMID: 1730755
Holt GD et al. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J Cell Biol 104:1157-64 (1987). PubMed PMID: 3571327
Snow CM et al. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J Cell Biol 104:1143-56 (1987). PubMed PMID: 2437126
General / background references:Dintzis SM et al. Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor. J Biol Chem 269:12159-66 (1994). PubMed PMID: 8163521
MacDonald RG et al. Serum form of the rat insulin-like growth factor II/mannose 6-phosphate receptor is truncated in the carboxyl-terminal domain. J Biol Chem 264:3256-61 (1989). PubMed PMID: 2536739
Featherstone C et al. A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo. J Cell Biol 107:1289-97 (1988). PubMed PMID: 2459127
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
IgM purified
Buffer System:
0.05% Sodium Azide
IgM purified
Detects nuclear pore-O-linked glycoprotein
Mammal, Rat, Xenopus, Yeast

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