SM5013P Nuclear Pore-O-Linked Glycoprotein antibody

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Mouse anti Amphibian, Rat, Yeast Nuclear Pore-O-Linked Glycoprotein RL1


Product Description for Nuclear Pore-O-Linked Glycoprotein

Mouse anti Amphibian, Rat, Yeast Nuclear Pore-O-Linked Glycoprotein RL1.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Nuclear Pore-O-Linked Glycoprotein

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms Nuclear Pore Marker
Presentation Purified
Reactivity Amph, Rt, Ye
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone RL1
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Isotype control SM13P
Application Immunoprecipitation.
Immunofluorescence: 1/100, staining of NPC O-linked glycoproteins with SM5013P results in exclusive labeling of the NPC proteins on a wide variety of mammalian cells as well as S. cerevisiae and Xenopus. Labeling occurs exclusively at the NPC with most of the labeling at the cytoplasmic and nucleoplasmic margins
Western Blot: 1/1000,detects up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa.
Background Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import. Reacts with Amphibian, Rat and Yeast.
General Readings
  1. J. Cell Biol., 104: 1157-1164, 1987.
  2. J. Cell Biol., 104:1143-1156, 1987.
  3. J. Cell Biol., 107: 1289-1297, 1988.
  4. Hum. Mol. Genet., Vol 12: 1847-1863, Aug 2003
  5. J. Cell Biol., Vol 156: 53, Jan 2002
  6. PNAS, Aug 2006; 103: 11952-11957.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS containing 0.05% sodium azide as preservative.
Liquid purified IgG fraction.
This antibody detects nuclear pore-O-linked glycoprotein from rat, amphibian and yeast.
Microinjected SM5013P inhibits both protein import and RNA export in Xenopus oocytes.
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