AM08257PU-S Nuclei (Nuclear Marker) antibody

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0.1 mg / €320.00
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Mouse anti Human, Primate Nuclei 235-1


Product Description for Nuclei

Mouse anti Human, Primate Nuclei 235-1.
Properties: (Nuclear Marker)
Presentation: Purified
Product is tested for Paraffin Sections, Immunoprecipitation, Frozen Sections, Immunocytochemistry/Immunofluorescence.

Properties for Nuclei

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Human Nuclear Antigen
Presentation Purified
Reactivity Hu, Prim
Applications C, ICC/IF, IP, P
Clonality Monoclonal
Clone 235-1
Host Mouse
Isotype IgG1
Molecular weight 70kda and 80kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Nuclei of human myeloid leukemia biopsy cells.
Property Nuclear Marker
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunofluorescence: 0.5-1 µg/ml. 
Immunoprecipitation: 0.5-1 μg/500 µg protein lysate. 
Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.25-0.5 µg/ml for 30 minutes at RT. It is recommended that the cells are permeabilized with 0.1 - 0.4% Triton X100.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin 
Sections: 0.5-1 µg/ml for 30 minutes at RT. 
Recommended Positive Control: Tonsil.
Background Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
Concentration 0.2 mg/ml
General Readings
  1. Rosario Sanchez-Pernaute et. al. Parthenogenetic dopami ne neurons from primate embryonic stem cells restore function in experimental Pa rkinson's disease. Brain. 2008; 131(8): 2127–2139.
  2. Glaser R, Lu MM, Narula N, Epstein JA. Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation. 2002 Jul 2;106(1):17-9. PubMed PMID: 12093763.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
Species Reactivity
Species reactivity (tested):
Human, Primates.
This Monoclonal Antibody is part of a new panel of rea gents, which recognizes subcellular organelles or compartments of Human cells.
These marker s may be useful in identification of these organelles in cells, tissues, and biochemica l preparations. Monoclonal Antibody 235-1 recognizes an antigen associated with the nuclei in Human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear ma rker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Cellular Localization: Nuclei. 
Negative Species: Mouse, Rat, Chicken.

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