NB100-78524 Nuclei antibody

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Mouse anti Human Nuclei 235-1


Product Description for Nuclei

Mouse anti Human Nuclei 235-1.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections.

Properties for Nuclei

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Reactivity Hu
Applications C, ICC/IF, IP, P
Clonality Monoclonal
Clone 235-1
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

CEM human T-cell leukemia cells
Add. information Research Areas: DNA Repair, Stem Cell Markers
Application immunofluorescence, Immunohistochemistry-Frozen, immunoprecipitation, Recommended Usage: Each lot of this antibody is quality control tested by Immunofluorescence staining. For immunofluorescence microscopy, suggested working dilution(s): Use a dilution range of 2-4 microgram/ml. For immunoprecipitation, this antibody can be used at 2-4 mug /1x107 cell equivalents. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application., immunofluorescence , immunoprecipitation , Immunohistochemistry-Frozen
Background Ku, is a heterodimeric DNA end binding complex composed of 70 KD and 80 kD subunits, serves as the DNA binding component of the DNA-dependent protein kinase (DNA-PK). Ku binds to double-stranded DNA breaks (DSBs), activates the catalytic subunit (DNA-PKcs) when DNA is bound, and is essential in DSB repair and V(D)J recombination. Clone 235-1 has been reported to bind the holoprotein (70KD and 80KD heterodimer) and both chains are need for the antigen to be recognized by this antibody. Clone 235-1 has been shown to be useful for Immunoprecipitation and Immunofluorescence staining. This clone is not suitable for Western blotting.
General Readings
  1. Walker, J. R., et al. 2001. Nature 412:607.2. Mari, P.-O., et al. 2006. Proc. Nat. Acad. Sci. 103:18597.3. Fu, X. D., et al. 1990. Nature 343:437.
Storage Store at 4C. Do not freeze.
Affinity purified
Buffer System:
0.09% Sodium Azide
Affinity purified
Aff - Purified
Mouse Monoclonal to Ku (235-1)

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