AP21353BT-N Nucleoside Monophosphate Kinase antibody

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1 ml / €340.00
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Rabbit anti Bovine Nucleoside Monophosphate Kinase


Product Description for Nucleoside Monophosphate Kinase

Rabbit anti Bovine Nucleoside Monophosphate Kinase.
Presentation: Biotin
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Nucleoside Monophosphate Kinase

Product Category Primary Antibodies
Quantity 1 ml
Synonyms NMPK, Nucleoside monophosphokinase
Presentation Biotin
Reactivity Bov
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Nucleoside Monophosphate Kinase isolated and purified from Bovine liver.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
Recommended Dilutions:
Non-precipitating antibody-binding techniques: 1/200-1/2,000.
Background Nucleoside monophosphate (NMP) kinases are ubiquitous enzymes involved in the biosynthesis of nucleotides. Each enzyme catalyses the synthesis of a nucleoside diphosphate that is, in turn, converted to a nucleoside triphosphate by a non-specific nucleoside diphosphate kinase. In prokaryotes, there are five NMP kinases, one for the phosphorylation of each NMP, whereas in eukaryotic organisms, the phosphorylation of both uridine monophosphate (UMP) and cytidine monophosphate (CMP) is carried out by a bifunctional UMP/CMP kinase (Liljelund and Lacroute, 1986).
Concentration 10.0 mg/ml
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Molar radio:
Bovine/IgG ~ 5.3
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
Lyophilized hyperimmune IgG fraction
Nucleoside Monophosphate Kinase from Bovine liver.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.

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