BM5527 NUP153 antibody

See related secondary antibodies

Search for all "NUP153"

5 ml / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Amphibian, Human, Mouse, Rat, Xenopus NUP153 nup7A8

BM5527

Product Description for NUP153

Mouse anti Amphibian, Human, Mouse, Rat, Xenopus NUP153 nup7A8.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for NUP153

Product Category Primary Antibodies
Target Category
Quantity 5 ml
Synonyms 153 kDa nucleoporin, Nuclear pore complex protein Nup153, Nucleoporin Nup153
Presentation Supernatant
Reactivity Amph, Hu, Ms, Rt, Xen
Applications C, ICC/IF, IP, WB
Clonality Monoclonal
Clone nup7A8
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P49790
Immunogen:
Nuclear matrix protein fraction prepared from Rat liver nuclei
GeneID:
9972
Application Immunohistochemistry / Immunofluorescence Microscopy on Frozen Sections: 1/5-1/10 (also after fixation with paraformaldehyde).
Immunoblotting (Western blot): 1/30-1/50 (ECL method).
Immunoprecipitation.
Incubation Time: 1h at RT.
Background Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex specific glycoproteins characterized by cytoplasmically oriented O linked N acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. Nup153 has three distinct domains: a N terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C terminal region containing multiple XFXFG repeats. Nup153 is a possible DNA binding subunit of the nuclear pore complex (NPC). The repeat containing domain may be involved in anchoring components of the pore complex to the pore membrane.
General Readings
  1. Cordes VC, Reidenbach S, Köhler A, Stuurman N, van Driel R, Franke WW. Intranuclear filaments containing a nuclear pore complex protein. J Cell Biol. 1993 Dec;123(6 Pt 1):1333-44. PubMed PMID: 8253834. (Free PMC Article available)
  2. Cordes VC, Gajewski A, Stumpp S, Krohne G. Immunocytochemistry of annulate lamellae: potential cell biological markers for studies of cell differentiation and pathology. Differentiation. 1995 Apr;58(4):307-12. PubMed PMID: 7641981.
  3. Cordes VC, Reidenbach S, Franke WW. Cytoplasmic annulate lamellae in cultured cells: composition, distribution, and mitotic behavior. Cell Tissue Res. 1996 May;284(2):177-91. PubMed PMID: 8625385.
  4. Hase ME, Cordes VC. Direct interaction with nup153 mediates binding of Tpr to the periphery of the nuclear pore complex. Mol Biol Cell. 2003 May;14(5):1923-40. PubMed PMID: 12802065. (Free PMC Article available, 9 images available)
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.

Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Hybridoma Culture Supernatant
Supernatant
Specificity
Specificity:
The antibody recognizes the nuclear pore complex (NPC) protein nup153 (190 kD), present on the inner aspect of NPC with attached filament bundles.
It is also reactive with the soluble form of nup153 found e.g. during meiotic metaphase. Epitope in Rat liver was mapped to N-terminal domain (aa439-611; cf. Hase & Cordes, 2003).
Species:
Human, Mouse, Rat, Kangaroo Rat, Amphibia (Xenopus, Pleurodeles).
Gene ID 9972
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for NUP153 (3 products)

Catalog No. Species Pres. Purity   Source  

NUP153

NUP153 Human
  Abnova Taiwan Corp.

NUP153

NUP153 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

NUP153

NUP153 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.
  • LinkedIn