NB300-524 O-GlcNAc (Golgi Apparatus Marker) antibody

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0.1 ml / €400.00
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Mouse anti Bovine, Drosophila, Fish, Human, Mouse, Rat, Xenopus, Virus O-GlcNAc RL2

NB300-524

Product Description for O-GlcNAc

Mouse anti Bovine, Drosophila, Fish, Human, Mouse, Rat, Xenopus, Virus O-GlcNAc RL2.
Properties: (Golgi Apparatus Marker)
Presentation: Ig Fraction
Product is tested for ChIP assay, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for O-GlcNAc

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Ig Fraction
Reactivity Bov, Dros, Fi, Hu, Ms, Rt, Vi, Xen
Applications ChIP, E, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone RL2
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Property Golgi Apparatus Marker
Application immunofluorescence, immunohistochemistry, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, Immunofluorescence, Western Blot, Immunoprecipitation, and Immunohistochemistry This antibody recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. Immunofluorescence staining of the NPC with this antibody primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus., immunohistochemistry , immunofluorescence 1:100, immunoprecipitation Assay dependent, Western Blot 1:1,000, Immunohistochemistry-Paraffin 1:200
Background Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC?s. NPC?s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.
General Readings This product has been used in:Han I et al. Interaction of the transcription factor Sp1 with the nuclear pore protein p62 requires the C-terminal domain of p62. J Cell Biochem 68:50-61 (1998). PubMed PMID: 9407313
Sterne-Marr R et al. O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import. J Cell Biol 116:271-80 (1992). PubMed PMID: 1730755
Holt GD et al. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J Cell Biol 104:1157-64 (1987). PubMed PMID: 3571327
Snow CM et al. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J Cell Biol 104:1143-56 (1987). PubMed PMID: 2437126
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
IgG purified
Buffer System:
0.05% Sodium Azide
Purity:
IgG purified
State:
Ig Fraction
Specificity
Specificity:
Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues.
Species:
Human, Mouse, Rat
Gene ID 8473

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