NB300-614 O-GlcNAc antibody

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0.1 mg / €380.00

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Mouse anti O-GlcNAc HGAC85


Product Description for O-GlcNAc

Mouse anti O-GlcNAc HGAC85.
Presentation: Ig Fraction
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for O-GlcNAc

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms O-linked N-acetylglucosamine
Presentation Ig Fraction
Applications E, ICC/IF, IP, WB
Clonality Monoclonal
Clone HGAC85
Host Mouse
Isotype IgG3
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Heat killed, pepsin treated group A streptococci.
Application ELISA, immunofluorescence, immunoprecipitation, Western Blot, Immunofluorescence: use at 10 ug/ml Western Blot: use at 1 ug/ml By Western blot, this antibody detects several proteins representing O-GlcNAc glycoproteins. Immunofluorescence staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties., ELISA, immunofluorescence, immunoprecipitation, Western Blot
Background Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling.
Concentration 2 mg/ml
General Readings This product has been used in:Zhang X & Bennett V Identification of O-linked N-acetylglucosamine modification of ankyrinG isoforms targeted to nodes of Ranvier. J Biol Chem 271:31391-8 (1996). PubMed PMID: 8940148
Turner JR et al. Cytologic assessment of nuclear and cytoplasmic O-linked N-acetylglucosamine distribution by using anti-streptococcal monoclonal antibodies. Proc Natl Acad Sci U S A 87:5608-12 (1990). PubMed PMID: 2116002
Nahm MH et al. Monoclonal antibodies to streptococcal group A carbohydrate. I. A dominant idiotypic determinant is located on Vk. J Immunol 129:1513-8 (1982). PubMed PMID: 6180013
General / background references:Holt GD et al. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J Cell Biol 104:1157-64 (1987). PubMed PMID: 3571327
Storage Store at -20C. Avoid freeze-thaw cycles.
Buffer System:
0.05% Sodium Azide
Ig Fraction
Recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins
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