SM5014P O-GlcNAc antibody

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Mouse anti O-GlcNAc RL2

Product Description for O-GlcNAc

Mouse anti O-GlcNAc RL2.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for O-GlcNAc

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms O-linked N-acetylglucosamine
Presentation Purified
Applications ICC/IF, IP, P, WB
Clonality Monoclonal
Clone RL2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Pore complex-lamina fraction purified from Rat liver nuclear envelopes.
Isotype control AM03095PU-N
Application Immunofluorescence: 1/100, primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus.
Immunohistochemistry on Paraffin Embedded Sections: 1/200.
Western Blot: 1/1000, recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. 
Dot Blot: 1/800.
Background Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.
Concentration 2.0 mg/ml
General Readings
  1. Am J Physiol Endocrinol Metab, Mar 2007; 292: E884 - E890.
  2. J. Biol. Chem., Oct 2007; 282: 31038 - 31045.
  3. Endocrinology Sept 2005 doi:10.1210/en.2005-0523
  4. JBC Papers in Press, manuscript M509481200, Oct 2005
  5. IOVS, 44(9): 3802-3809, 2003.
  6. J. Cell Biol., 104: 1157-1164, 1987.
  7. J. Cell Biol., 104:1143-1156, 1987.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A Chromatography
Buffer System:
PBS buffer with 0.05% Sodium Azide as preservative
Liquid purified IgG fraction
This antibody detects Nuclear Pore Complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins form Human, Mouse, Virus and Rat tissues.

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