discontinued

SM5136P O-GlcNAC antibody

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0.1 mg / €420.00

Quick Overview

Mouse anti O-GlcNAC HGAC85

SM5136P

Product Description for O-GlcNAC

Mouse anti O-GlcNAC HGAC85.
Presentation: Ascites
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for O-GlcNAC

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms O-linked N-acetylglucosamine
Presentation Ascites
Applications E, ICC/IF, IP, WB
Clonality Monoclonal
Clone HGAC85
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Heat killed, pepsin treated group A streptococci.
Application ELISA.
Immunoprecipitation.
Western Blot: 1 µg/ml, this antibody detects several proteins representing O-GlcNAc glycoproteins.
Immunofluorescence: 10 µg/ml, staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties.
Background Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling.
Concentration 2.0 mg/ml
General Readings
  1. Diabetes Vol. 53, June 2004
  2. J. Cell Biol. 104: 1157-1164, 1987.
  3. Proc. Natl. Acad. Sci. 57: 5608-5612, 1990.
  4. J. Immun., 129(4): 1513-1518, 1982.
  5. J. Biol. Chem., 271(49): 31391-31398, 1996.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS containing 0.05% Sodium Azide as preservative.
DO NOT USE WITH DILUENTS CONTAINING GLYCOSYLATED PROTEINS.
State:
Liquid diluted Ascites
Ascites
Specificity
Specificity:
This antibody recognizes beta-1,3 linked O-linked N-Acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins.

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