Product Description for O-GlcNAC
Mouse anti O-GlcNAC HGAC85.
Presentation: Ascites
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Presentation: Ascites
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Properties for O-GlcNAC
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | O-linked N-acetylglucosamine |
| Presentation | Ascites |
| Applications | E, ICC/IF, IP, WB |
| Clonality | Monoclonal |
| Clone | HGAC85 |
| Host | Mouse |
| Isotype | IgG3 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Application | ELISA. Immunoprecipitation. Western Blot: 1 µg/ml, this antibody detects several proteins representing O-GlcNAc glycoproteins. Immunofluorescence: 10 µg/ml, staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties. |
| Background | Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling. |
| Concentration | 2.0 mg/ml |
| General Readings |
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| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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Accessory Products
Recommended Secondary Antibodies for O-GlcNAC (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
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|
Rabbit | AP | C, E, WB | ||||
| Acris Antibodies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | Biotin | C, E, WB | ||||
| Acris Antibodies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | FITC | E, ICC/IF | ||||
| Acris Antibodies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
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|
Rabbit | HRP | C, E, WB | ||||
| Acris Antibodies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | Texas Red | ICC/IF | ||||
| Acris Antibodies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Rabbit | PE | F | ||||
| Acris Antibodies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Goat | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy2 | |||||
| Acris Antibodies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy3 | |||||
| Acris Antibodies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy5 | |||||
| Acris Antibodies GmbH | |||||||
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