AP26410PU-N Occludin (358-504) antibody

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0.1 mg / €380.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Guinea Pig anti Canine, Human, Mouse, Rat Occludin

AP26410PU-N

Product Description for Occludin

Guinea Pig anti Canine, Human, Mouse, Rat Occludin.
Properties: (358-504)
Presentation: Purified
Product is tested for Western blot / Immunoblot, Immunocytochemistry/Immunofluorescence, Frozen Sections.

Properties for Occludin

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms OCLN
Presentation Purified
Reactivity Can, Hu, Ms, Rt
Applications C, ICC/IF, WB
Clonality Polyclonal
Host Guinea Pig
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q16625
Immunogen:
Recombinant Human Occludin (aa 358-504)
GeneID:
100506658
Property 358-504
Application Immunohistochemistry on Frozen Sections: The typical starting working dilution is 1/50.
Immunoflourescence: The typical starting working dilution is 1/50.
Western blot: The typical starting working dilution is 1/50.
Background Occludin is an integral membrane protein specifically associated with tight junctions. These specialized regions of the plasma membrane prevent the diffusion of dissolved molecules between adjacent epithelial cells, seal off body cavities such as intestine or stomach lumen and prevent the diffusion of membrane proteins and glycolipids between the apical and basolateral regions of the plasma membrane. Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1).
Concentration 0.1 mg/ml
General Readings
  1. Van Itallie CM, Anderson JM. Occludin confers adhesiveness when expressed in fibroblasts. J Cell Sci. 1997 May;110 ( Pt 9):1113-21. PubMed PMID: 9175707.
Storage

Store undiluted at 2-8°C.
Shelf life: one year from despatch.

Format
Purification:
Protein A Chromatography
Buffer System:
PBS
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.1% BSA
State:
Liquid 0.2 µm filtered Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse, Rat, Canine.
Specificity
Specificity:

The polyclonal antibody reacts with Occludin.

Gene ID 100506658

Accessory Products

Proteins and/or Positive Controls

Proteins for Occludin (6 products)

Catalog No. Species Pres. Purity   Source  

Occludin

Occludin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Occludin

Occludin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Occludin

Occludin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Occludin

Occludin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Occludin

Occludin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Occludin

Occludin Human Purified
  Novus Biologicals Inc.

Positive controls for Occludin (3 products)

Catalog No. Species Pres. Purity   Source  

Occludin Lysate

Western Blot: Occludin Lysate [NBL1-13904] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for OCLN
  Novus Biologicals Inc.

OCLN 293T Cell Transient Overexpression Lysate(Denatured)

OCLN 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

OCLN overexpression lysate

OCLN overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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