AM03173PU-S OCT3/4 antibody

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0.5 ml / €420.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human OCT3/4 NRG1.1

Product Description for OCT3/4

Mouse anti Human OCT3/4 NRG1.1.
Presentation: Purified
Product is tested for Paraffin Sections.

Properties for OCT3/4

Product Category Primary Antibodies
Target Category
Quantity 0.5 ml
Synonyms OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
Presentation Purified
Reactivity Hu
Applications P
Clonality Monoclonal
Clone NRG1.1
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q01860
Immunogen:
BALB/C mice injected with synthetic peptide of Human OCT-4 origin.
GeneID:
5460
Isotype control AM03110PU-N, SM12P
Application Immunohistochemistry on Formalin fixed paraffin embedded tissue sections: 1/25-1/50 in an ABC method.
Positive Control: Seminoma.
Background OCT-4 (also known as POU5F1), a transcription factor that has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and primordial germ cells, has been proposed as a useful marker for Germ Cell Tumors (GCTs) that exhibit features of pluripotentiality, specifically seminoma / dysgerminoma / germinoma and embryonal carcinoma.
Protocols Staining Protocol
We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
General Readings
  1. Cheng et al. J Pathol 211: 1, 2007.
  2. Jung et al. Appl Immunohistochem Mol Morph 14: 273, 2006.
  3. Sung et al. Human Pathol. Jun;37(6):662-7, 2006.
  4. Jones et al. Am J Surg Pathol. Nov,30(11):1427-31, 2006.
  5. Hattab et al. Am J Surg Pahtol. 29(3):368-371, 2005.
  6. Lau et al. Adv in Anat Path. 13(2):76-79, 2006.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
≤  0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
OCT-4 immunostaining has been shown to be a sensitive and specific marker for seminomatous / (dys)germinomatous tumours and in embryonal carcinoma variants of non-seminomatous GCTs, whether in primary gonadal or extragonadal sites or in metastatic lesions. 
Cellular Localization: Nuclear.
Gene ID 5460
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for OCT3/4 (5 products)

Catalog No. Species Pres. Purity   Source  

OCT3/4 (transcript variant 1)

OCT3/4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

OCT3/4

OCT3/4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

OCT3/4

OCT3/4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

OCT3/4

OCT3/4 Human
  Abnova Taiwan Corp.

OCT3/4

OCT3/4 Human
  Abnova Taiwan Corp.

Positive controls for OCT3/4 (1 products)

Catalog No. Species Pres. Purity   Source  

OCT4 Lysate

Western Blot: OCT4 Lysate [NBL1-14620] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for POU5F1
  Novus Biologicals Inc.
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