MO15002-50 Oligodendrocyte Marker O4 antibody

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50 µg / €300.00
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Mouse anti Chicken, Human, Mouse, Rat Oligodendrocyte Marker O4 O4

Product Description for Oligodendrocyte Marker O4

Mouse anti Chicken, Human, Mouse, Rat Oligodendrocyte Marker O4 O4.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for Oligodendrocyte Marker O4

Product Category Primary Antibodies
Quantity 50 µg
Presentation Purified
Reactivity Chk, Hu, Ms, Rt
Applications ICC/IF
Clonality Monoclonal
Clone O4
Host Mouse
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Neuromics Antibodies

Datasheet Extract

Immunogen
Immunogen:
White matter of corpus callosum from Bovine brain.
Application Immunohistochemistry: 1-3 µg/mL fixed; 5 µg/mL unfixed, frozen. 
Antiserum was used on fixed and unfixed tissue. This antibody can be used with the appropriate secondary reagents at 1 - 3 µg/mL to detect Oligodendrocyte marker O4 in fixed cells. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 min., and then blocked with 10% normal donkey serum and 1% BSA in PBS at room temperature for 45 min. After blocking, cells were incubated with diluted primary antibody overnight at 4° C and then with Rodamine Red coupled anti-mouse IgM or other appropriate secondary antibody at room temperature in the dark for an hour. Between each step, cells were washed with PBS + 0.1% BSA. This antibody can also be used in unfixed, shock frozen tissue at the concentration of 5 µg/mL.
Background Oligodendrocyte Markers O4 will react with late OL progenitors, by reacting with an unidentified sulfated glycolipid antigen called POA (Proligodendrocyte Antigen). It also reacts with OLs that have entered terminal differentiation, in which case it reacts with sulfated galactosylcerebroside.
Format
State:
Lyophilized 0.2 µm filtered solution in phosphate-buffered saline (PBS) with 5% trehalose
Purified
Reconstitution:
Reconstitute w/ sterile PBS. If 50 ul is used, the concentration will be 1 mg/ml.
Species Reactivity
Species reactivity (tested):
Rat, Human, Mouse, Chicken.
Specificity
Specificity:
Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. The monoclonal antibody O4 reacts with an unidentified antigen that appears on the surface of oligodendrocyte progenitors. It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage.

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