AP03009PU-N Orf1 antibody

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0.1 mg / €260.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Orf1

Product Description for Orf1

Rabbit anti Orf1.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Orf1

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
Application Suitable for Western Blotting under Reducing conditions (0.2-1 μg/ml).
Background Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
Concentration 1.0 mg/ml
General Readings
  1. Prochazkova K, Osicka R, Linhartova I, Halada P, Sulc M, Sebo P. The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC. J Biol Chem. 2005 Feb 4;280(5):3251-8. Epub 2004 Nov 2. PubMed PMID: 15525636.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein-A affinity chromatography.
Buffer System:
PBS, pH 7.4 with 15 mM sodium azide as preservative.
Liquid purified IgG fraction (> 95% pure by SDS-PAGE).
Aff - Purified
The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.

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