DM3158 P105 antibody

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Mouse anti Human P105 B301


Product Description for P105

Mouse anti Human P105 B301.
Presentation: Supernatant
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for P105

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms PANA, Proliferation Associated Nuclear Antigen, Proliferation Marker
Presentation Supernatant
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone B301
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Activated peripheral blood nuclei
Mol. Wt. of antigen: dimeric form 105 kD, monomeric form 41 kD
Application Western blot (1:100 - 1:200)
Immunohistochemistry (1:50 - 1:100). This antibody can be used on formalin-fixed, paraffin embedded tissue sections. FFPE tissue sections require pepsin or trypsin digestion. Prolonged fixation in buffered formalin can destroy the epitope. It is recommended that this product be used on frozen tissue sections or specimens.
Recommended positive control: human tonsil or lymph node.
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.25 mg/ml
General Readings 1. Clevenger, CV., Epstein, AL., Exp.Cell.Res., 151:194-207, 1984.
2. Clevenger, CV., Epstein, AL.,
J. of Histochemistry and Cytochemistry, 32: 757-765, 1984.
3. Clevenger, CV., et. al., Cytometry 6:208-214, 1985.
4. Bauer, KD., et.al., Canc.Res. 46:2428-2434, 1986.
5. Clevenger, CV., et.al., Cytometry 8:280-286, 1987.
6. Clevenger, CV., et.al., J. Cellular Physiology 130:336-343, 1987.
7. Fitzgibbons, PL., et.al., Am. J. of Clin. Path., 89:640-644, 1988.
8. Turner, RR., et.al., Arch.of Path. and Lab. Med., 113:907-911, 1989.
9. Yoneumura, Y., et.al., Can
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire contents of the vial at once, since the diluted solution may have reduced stability.. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies.
The p105 protein, found in all human cells in a dimeric form (105 kD) and monomeric form (41 kD), is a proliferation associated nuclear antigen that is absent in non-cycling cells in the G0 phase. This protein is important in the synthesis and transport of RNA in the cell regulation cycle. During G2 and mitosis, this protein is increased possibly due to the unmasking of nucleolar antigens, resulting in large accumulations of this protein in the mitotic cytoplasm.
This antibody reacts with human samples. Others not tested.

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