AM05216PU-N p110 (mitochondrial membrane protein) antibody

See related secondary antibodies

Search for all "p110 (mitochondrial membrane protein)"

0.1 mg / €360.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Monkey p110 (mitochondrial membrane protein) 2G2

AM05216PU-N

Product Description for p110 (mitochondrial membrane protein)

Mouse anti Human, Monkey p110 (mitochondrial membrane protein) 2G2.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for p110 (mitochondrial membrane protein)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu, Mky
Applications ICC/IF, WB
Clonality Monoclonal
Clone 2G2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Skeletal fracrtion of HeLa-S3 cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot (2-10 ug/ml)
Immunofluorescence.
Background P110 is a human mitochondrial protein with a molecular weight of 110 kDa. Close examination of the staining pattern in HeLa and Fanconi's Anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. The epitope targeted may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells.
Concentration Lot specific
Product Citations
Originator or purchased from resellers:
  1. Criollo A, Maiuri MC, Tasdemir E, Vitale I, Fiebig AA, Andrews D, et al. Regulation of autophagy by the inositol trisphosphate receptor. Cell Death Differ. 2007 May;14(5):1029-39. Epub 2007 Jan 26. PubMed PMID: 17256008.
General Readings
  1. Paulin-Levasseur M, Chen G, Larivière C. The 2G2 antibody recognizes an acidic 110-kDa human mitochondrial protein. Histochem J. 1998 Sep;30(9):617-25. PubMed PMID: 9870762.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
PBS
Preservatives:
0.08% Sodium Azide
Purity:
Purified
State:
Liquid purified IgG fraction from Ascites
Purified
Specificity
Specificity:
Reacts with Human and Monkey mitochondria.
It does not react with Chicken, Mouse or Rat.  
After stabilization with Aldehyde- or Alcohol-based fixation protocols that optimize the preservation of cytoskeletal components, the epitope targeted by the 2G2 antibody may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in Human cells.
The Mouse monoclonal P110 antibody labels mitochondria in Human cells, as assessed by double staining with either Rhodamine 123 or a polyclonal antibody to mitochondrial matrix HSP-60 proteins. The P110 antigen has an approximate isoelectric point of 6.5 that copartitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. The P110 staining pattern in HeLa and Fanconi's anaemia cells reveals differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi's anaemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules.

Accessory Products

  • LinkedIn