AP00553PU-N p14 ARF antibody

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Rabbit anti Human p14 ARF

Product Description for p14 ARF

Rabbit anti Human p14 ARF.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for p14 ARF

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms DP3095, p14ARF
Presentation Purified
Reactivity Hu
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

A synthetic peptide from the C-terminus of human p14ARF.
Mol. Wt. of Antigen: 14 kDa
Application Immunofluorescence.
Immunohistology (formalin/paraffin): use Ab at 5-10 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: HeLa Cells, Cervical Carcinoma, or Breast Fibroadenoma.
Background The INK4a-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. p14ARF binds to mdm2 and promotes the rapid degradation of mdm2 proteinrequired for mdm2 modification and concurrent p53 stabilization and accumulation. This interaction is mediated by the exon 1β-encoded Nterminaldomain of p14ARF and a C-terminal region of
mdm2. Deletion of the ARF-INK4a locus simultaneously impairs both the INK4a-cyclin D/cdk4-RB and the ARF-mdm2-p53 pathways.
Concentration 1 mg/ml
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.
Liquid IgG
This antibody detects p14ARF.
It may show some additional bands with MW much higher than 14 kDa. However, the p14ARF area is relatively clean which permits an easy identification of the p14ARF band.
Cellular Localization: Nuclear.

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