DP3095 p14 ARF antibody

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0.5 mg / €270.00

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Rabbit anti Human p14 ARF


Product Description for p14 ARF

Rabbit anti Human p14 ARF.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for p14 ARF

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms p14ARF
Presentation Purified
Reactivity Hu
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

A synthetic peptide from the C-terminus of human p14ARF.
Application Immunofluorescence. Immunohistology (formalin/paraffin): use Ab at 5-10µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: HeLa Cells, Cervical Carcinoma, or Breast Fibroadenoma. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 1 mg/ml
General Readings
  1. Zhan
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
The INK4a-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. Cellular Localization: nuclear.

Notes: Ab-4 may show some additional bands with MW much higher than 14kDa. However, the p14ARF area is relatively clean which permits an easy identification of the p14 ARF band. For optimun results, immunoprecipitate p14 ARF with Ab-4 and then Western blot with Ab-2.

Mol. Wt of Antigen: 14kD.

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