discontinued

SP6370P p70 S6 Kinase (p70S6K) non pSer434 antibody

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50 µg / €250.00

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Rabbit anti Human, Mouse, Rat p70 S6 Kinase (p70S6K) non

Product Description for p70 S6 Kinase (p70S6K) non

Rabbit anti Human, Mouse, Rat p70 S6 Kinase (p70S6K) non.
Properties: pSer434
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for p70 S6 Kinase (p70S6K) non

Product Category Primary Antibodies
Quantity 50 µg
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications E, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer AnaSpec

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide of human p70 S6K surrounding Serine 434.
AA Sequence:
VAPSVLE
Property pSer434
Application ELISA (0.1 - 1.0 µg/ml).
Western blot (0.5 - 2.0 µg/ml).
Immunoprecipitation (3.0 - 5.0 µg/extract from 10e7 cells).
Background The protein p70 S6 kinase is critical for cell cycle progression and cell survival. In response to mitogen stimulation, p70 S6 kinase activation up-regulates ribosomal biosynthesis and enhances the translational capacity. The p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome. This kinase was first characterized as an insulin/mitogen-activated protein kinase, whose major known substrate is the 40 S ribosomal subunit protein S6. The p70 S6 kinase is activated by diverse stimuli through a multi-site phosphorylation such as Thr-252 and Ser-434. In Alzheimer's disease (AD), p70 S6 kinase activation is associated with PHF-tau (hyperphosphorylated tau) accumulation. In non-neuronal cells, p70 S6 kinase have been shown to regulate actin polymerization.
Concentration 0.2 mg/ml
General Readings
  1. Avruch, J. Mol. Cell Biochem. 1998 182, 31-48.
  2. Qing-Ping Weng et al. J Biol Chem, 1998 273(26), 16621-16629.
  3. An, WL et al. Am J Pathol. 2003 163(2), 591-607.
  4. Price, D, et al J. Biol. Chem., 1991 266(25), 16281-16284.
  5. Raymond C et al Neuroscience. 2002 109, 531-6.
Storage Store the antibody at 2 - 8 °C or (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Epitope affinity chromatography
Buffer System:
1x PBS (pH 7.4) containing 0.05 % sodium azide
State:
Liquid Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody reacts specifically with p70S6K. It was evaluated by Western blot and ELISA. By Western blot, an immunoreactive band around 70 kDa can be detected in HEK293, 3T3 and HeLa whole cell lysates. This band can be blocked by immunizing peptide.
Species:
Human, Mouse, Rat.

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