NSB942 PAK1/PAK2/PAK3 antibody

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Rabbit anti Human PAK1/PAK2/PAK3

Product Description for PAK1/PAK2/PAK3

Rabbit anti Human PAK1/PAK2/PAK3.
Product is tested for Western blot / Immunoblot.

Properties for PAK1/PAK2/PAK3

Product Category Primary Antibodies
Quantity 0.1 ml
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PAK that contains threonine 423 (for PAK1). The sequence is conserved across many species for all three main isoforms of PAK.
Add. information Research Areas: Cancer
Application Western Blot, This antibody is suitable for use in Western blotting. , Western Blot 1:1,000
Background p21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK1, PAK2, and PAK3 (also known as a-PAK, g-PAK, and b-PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase-mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
General Readings Zang, M., et al. (2002) Interaction between active Pak1 and Raf-1 is necessary for phosphorylation and activation of Raf-1. J. Biol. Chem. 277(6):4395-4405.
Chong, C., et al. (2001) The mechanism of PAK activation. Autophosphorylation events in both regulatory and kinase domains control activity. J. Biol. Chem. 276(20):17347-17353.
del Pozo, M.A., et al. (2000) Adhesion to the extracellular matrix regulates the coupling of the small GTPase Rac to its effector PAK. EMBO J. 19(9):2008-2014.
Schurmann, A., et al. (2000) p21-activated kinase 1 phosphorylates the death agonist Bad and protects cells from apoptosis. Mol. Cell Biol. 20(2):453-461.
Zenke, F.T., et al. (1999) Identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity. J. Biol. Chem. 274(46):32565-32573.
Gatti, A., et al. (1999) Multisite autophosphorylation of p21-activated protein kinase gamma-PAK as a function of activation. J. Biol. Chem. 274(12):8022-8028.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
PAK 1 + 2 + 3 Phosphospecific [Thr423]

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