AM20217PU-N PAK4 (incl. pos. control) antibody

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0.1 mg / €430.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Canine, Human, Mouse, Rat PAK4 6C1

Product Description for PAK4

Mouse anti Canine, Human, Mouse, Rat PAK4 6C1.
Properties: (incl. pos. control)
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for PAK4

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms KIAA1142, PAK 4, PAK-4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4
Presentation Purified
Reactivity Can, Hu, Ms, Rt
Applications WB
Clonality Monoclonal
Clone 6C1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O96013
Immunogen:
Peptide conjugated to hemocyanin
Epitope: Peptide derived from the kinase activation loop.
GeneID:
10298
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Add. information Mol. weight: 60 kDa
Application Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
Included Positive Control: Cell lysate from untreated SW480 cells (See Protocols for more details).
Background p21 activated kinases (PAK) are regulated by the small GTP - binding proteins Rac and cdc42, which stimulate autophosphorylation and phosphorylation of downstream signaling proteins. PAK4 is activated upon autophosphorylation of Ser 474 in the kinase activation loop. PAK4 is frequently overexpressed in human tumor cell lines and might play an important role in oncogenesis.
Protocols Positive Control Provided.
Cell lysate from untreated SW480 cells


Description: Cell lysate from untreated SW480 cells, colorectal adenocarcinoma (Human)

Format: Lyophilized cell lysate from serum starved SW480 cells.

Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Storage For long-term storage, freeze lyophilizate upon arrival (-20°C).
Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year.
Avoid repeated freeze / thaw cycles.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Format
Purification:
Subsequent Ultrafiltration and Size Exclusion Chromatography.
Buffer System:
1ml PBS, containing 0.09% Sodium Azide/PEG and Sucrose.
State:
Lyophilized purified Ig fraction.
Purified
Reconstitution:
Restore with 1 ml H2O (15 min, RT).
Specificity
Specificity:
Recognizes p21 activated kinase 4 (PAK4).
Species:
Human, Mouse, Rat and Canine (Dog).
Gene ID 10298

Accessory Products

Proteins and/or Positive Controls

Proteins for PAK4 (16 products)

Catalog No. Species Pres. Purity   Source  

PAK4 (transcript variant 1)

PAK4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PAK4 (transcript variant 2)

PAK4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PAK4 (transcript variant 3)

PAK4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PAK4 (transcript variant 1)

PAK4 Human > 90 %
Preparation: .
Purity Detail: >90% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €139.00
  OriGene Technologies, Inc.

PAK4 (transcript variant 1)

PAK4 Human > 90 %
Preparation: .
Purity Detail: >90% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
0.1 mg / €978.00
  OriGene Technologies, Inc.

PAK4 (1-591, His-tag)

PAK4 Human Purified > 90 % by SDS PAGE E. coli
0.5 mg / €990.00
  Acris Antibodies GmbH

PAK4 (1-591, His-tag)

PAK4 Human Purified > 90 % E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

PAK4

PAK4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PAK4

PAK4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PAK4

PAK4 Human
  Abnova Taiwan Corp.

PAK4

PAK4 Human
  Abnova Taiwan Corp.

PAK4

PAK4 Human
  Abnova Taiwan Corp.

PAK4

PAK4 Human
  Abnova Taiwan Corp.

PAK4

PAK4 Human
  Abnova Taiwan Corp.

PAK4

PAK4 Human
  Abnova Taiwan Corp.

PAK4

SDS-Page: PAK4 Protein [NBC1-18517] - PAK4, 68.3 kDa (628 aa) with a purity of 90% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for PAK4 (2 products)

Catalog No. Species Pres. Purity   Source  

PAK4 Lysate

Western Blot: PAK4 Lysate [NBL1-14080] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PAK4
  Novus Biologicals Inc.

PAK4 Lysate

Western Blot: PAK4 Lysate [NBL1-14081] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PAK4
  Novus Biologicals Inc.
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