AM10028FC-N pan Cytokeratin antibody

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0.2 mg / €480.00
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Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat pan Cytokeratin AE1/AE3

AM10028FC-N

Product Description for pan Cytokeratin

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat pan Cytokeratin AE1/AE3.
Presentation: FITC
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Properties for pan Cytokeratin

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms Cytokeratin pan-reactive, pan Keratin
Presentation FITC
Reactivity Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt
Applications F, ICC/IF
Clonality Monoclonal
Clone AE1/AE3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human epidermal keratin.
Remarks:
Molecular Weight of Antigen: CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) CK8 (52.5). + CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) CK8 (52.5).
Isotype control AM03095PU-N, SM10F (for use in human samples), SM20F (for use in rat samples)
Application Immunofluorescence: 10-20 µg /ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4°C.
Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000) (Not tested in our lab).
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).
Concentration 0.2 mg/ml
General Readings Battifora, H., Sheibani, K., Tubbs, R.R., Kopinski, M.I. and Sun, T.-T.: Anti-keratin antibodies in tumor diagnosis: Distinction between seminoma and embryonal carcinoma. Cancer 54, 843-848 (1984) 
Eichner, R., Bonitz, P. and Sun, T.-T.: Classification of epidermal keratins according to their immunoreactivity, isoelectric point and mode of expression. J. Cell. Biol. 98, 1388-1396 (1984) 
Garin-Chesa, P., Rettig, J. and Melamed, R.: Expression of cyto- keratin in normal and neoplastic colonic epithelial cells: Implications for cellular differentiation and carcinogenesis. Am. J. Surg. Pathol. 10, 829-835 (1986) 
Nelson, W.G., Battifora, H., Santana, H. and Sun, T.-T.: Specific keratins as molecular markers for neoplasms with a stratified epithelial origin. Cancer Res. 44, 1600-1603 (1984) 
Nelson, W.G. and Sun, T.-T.: The 50- and 58- kD keratin classes as molecular markers for stratified squamous epithelia: Cell culture studies. J. Cell Biol. 97, 244-251 (1983) 
Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982) 
Shi, S.-R., Goodman, M.L., Chan, A.K., Pilch, B.Z., Chen, L.B. and Sun, T.-T.: Immunohistochemical study of nasopharyngeal carcinoma using monoclonal anti-keratin antibodies. Am. J. Pathol. 117, 53-63 (1984) 
Spagnolo, D.V., Michie, S.A., Crabtree, G.S., Warnke, R.A. and Rouse, R.V.: Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am. J. Clin. Pathol. 84, 697-704 (1985) 
Sun, T.-T., Eichner, R., Cooper, D., Schermer, A., Nelson, W.G. and Weiss, R.A.: Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. The Cancer Cell 1, 169-176 (1984) 
Sun, T.-T., Eichner, R., A., Nelson, W.G. Tseng, S.C.G., Weiss, R.A., Jarvinen, M. and Woodcock-Mitchell, J.: Keratin classes: Molecular markers for different types of epithelial differentiation. J. Invest. Dermatol. 81, 109-115 (1983)
Sun, T.-T., Tseng, S.C.G., Huang, A.J.W., Cooper, D., Lynch, M.H., Weiss, R.A., Eichner, R., A. and Schermer, A.: Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments. The New York Acad. Sci. (E. Wang, D. Fischmann, R. Liem, and T.-T. Sun, eds.) 455, 307-329 (1985)
Tseng, S.C.G., Jarvinen, M., Nelson, W.G., Huang, J.-W., Woodcock-Mitchell, J. and Sun, T.-T.: Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30, 361-372 (1982) 
Woodcock-Mitchell, J., Eichner, R., Nelson, W.G. and Sun, T.-T.: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J. Cell Biol. 95, 580-588 (1982) 
Weiss, R.A., Eichner, R. and Sun, T.-T.: Monoclonal antibody analyis of keratin expression in epidermal diseases: A 48 kD and a 56 kD keratin as molecular markers for hyperproliferating keratinocytes. J. Cell Biol. 98, 1397-1406 (1984) 
Weiss, R.A., Guillet, G.Y., Freedberg, I.M., Small, E.-A., Farmer, E.R., Weiss, M. and Sun, T.-T.: The use of monoclonal antibody to keratin in human epidermal disease: Alterations in immunohistoche- mical staining pattern. J. Invest. Dermatol. 81, 2279-2286 (1983)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
FITC
Purification:
Protein A Chromatography
Buffer System:
PBS, pH 7.4
Preservatives:
0.05% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Monkey, Bovine, Porcine, Rat, Mouse, Rabbit and Chicken.
Specificity
Specificity:
AE 1/AE3 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors; reacts with all epithelium-derived tumors and their neoplasms. Decorates the majority of type I and type II keratins (formerly also designated cytokeratins). 
This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
This antibody AE 1/AE 3 recognizes Low Molecular Weight Cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40) of the acidic family and CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) and CK8 (52.5).
Cellular Localization: Cytoplasmic.

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