AM33205PU-N pan Cytokeratin antibody

See related secondary antibodies

Search for all "pan Cytokeratin"

1 ml / €950.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human pan Cytokeratin BS5

Product Description for pan Cytokeratin

Mouse anti Human pan Cytokeratin BS5.
Presentation: Purified
Product is tested for Paraffin Sections.

Properties for pan Cytokeratin

Product Category Primary Antibodies
Quantity 1 ml
Synonyms Cytokeratin pan-reactive, pan Keratin
Presentation Purified
Reactivity Hu
Applications P
Clonality Monoclonal
Clone BS5
Host Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Immunohistochemistry on Paraffin Sections: 1/100-1/300. 
Positive Control: Tonsil, liver, appendix.
Background Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.
Protocols After de-paraffination and rehydration: 

1) Pretreatment: HIER pH9
2) Wash (TBS-Tween).
3) Primary antibody: Pan Cytokeratin 1:100-1:300, 30 min.
4) Wash (TBS-Tween).
5) 3 % H2O2, 10 min.
6) Wash (TBS-Tween).
7) HRP One-Step Polymer , 30 min
8) Wash (TBS-Tween).
9) Wash (TBS-Tween).
10) DAB high contrast Kit , 10 min
11) Wash in distilled water.
12) 0,5% CuSO4 -post enhancement, 5 min.
13) Wash in distilled water.
14) Counter staining in diluted Mayer, 1 min.
15) Bluing, 7 min in tap water.
16) Dehydration, clearing and mounting.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
0.03% Sodium Azide
Liquid purified IgG fraction
Human Pan Cytokeratin. Other species not tested.

Accessory Products

  • LinkedIn